ACQUITY UPLC FLR fluorescence detector: performance and precision for chromatographic analysis
The ACQUITY UPLC FLR fluorescence detector, developed by Waters, has been specifically designed to integrate with the ACQUITY UPLC system and meet the requirements of modern laboratories. It combines remarkable sensitivity and high selectivity, essential qualities for quantifying fluorescent compounds at very low concentrations, whether naturally fluorescent or labelled with tags.
This detector features a unique optical architecture that significantly reduces light scattering, allowing maximum signal transmission. Its high data rate of up to 80 Hz ensures fast acquisition, which is essential for high-throughput analyses. The low-volume axial flow cell focuses the excitation energy in a targeted manner, maximising light absorption and therefore signal response.
Thanks to its 2D and 3D detection modes, the user can easily determine the optimal wavelengths for excitation and emission. This flexibility is an asset for methodological development, characterisation of unknown compounds or confirmation of a peak of interest. The Spectral λ-λ mode, in particular, allows simultaneous visualisation of the excitation and emission spectra on a three-dimensional map.
The software interface, compatible with Empower™ and MassLynx™, provides simplified access to all instrument parameters. It effectively replaces the old physical controls with intuitive and clear navigation. This provides a comfortable user experience while ensuring total control over the instrument.
In practical applications, the FLR detector can, for example, detect anthracene at a detection limit (LOD) of 3.2 femtograms per column, demonstrating its ability to work at extremely low levels. Whether for the chromatography of water-soluble vitamins, toxicological analyses or the separation of compounds such as polycyclic aromatic hydrocarbons (PAHs), the detector maintains remarkable reproducibility and analytical resolution.
The ACQUITY UPLC FLR detector is an essential tool for laboratories seeking accurate, fast and reproducible results with high data quality and ultra-low detection limits.
