High-precision UV/VIS detection with the Agilent G1314A detector
The Agilent G1314A variable wavelength detector is a high-performance optical detection module integrated into the Agilent 1200 Infinity HPLC platform. It uses a dual-beam system combined with a deuterium lamp to cover the spectral range from 190 to 600 nm, which is essential for detecting compounds that absorb in the ultraviolet range. Its optical architecture includes a collecting lens, spherical mirrors, a stepper motor-driven diffraction grating for rapid wavelength selection, and a photodiode array that converts light signals into usable digital data.
Designed to meet the requirements of modern chromatography, the G1314A is optimised for isocratic and gradient analyses in HPLC. It offers an excellent signal-to-noise ratio with increased baseline stability, thanks in particular to its integrated electronic temperature control. This system minimises drift effects due to environmental variations, thereby improving the reproducibility of results.
This detector is compatible with various types of flow cells, such as standard, semi-micro, micro or high-pressure cells, each equipped with an RFID tag for electronic monitoring of critical parameters (volume, optical path length, maximum pressure, etc.). This technology ensures unambiguous identification of components, contributing to complete traceability of analyses in accordance with GLP standards.
The G1314A can be easily integrated into analytical chains via multiple interfaces: LAN, RS-232C, CAN and APG Remote. It is fully controllable via Agilent ChemStation software or the Instant Pilot interface, allowing precise control of parameters such as wavelength, signal intensity or lamp activation. The user can program spectral scans in stop-flow mode for online spectral analysis of peaks of interest.
Maintenance of the G1314A is facilitated by direct front access to the lamp and flow cell, making replacement quick and easy without the need for specialised tools. The module also includes diagnostic functions, leak detection with automatic shutdown, and electronic recording of errors and maintenance operations via RFID.
With its accuracy, stability and control features, the G1314A is the detector of choice for UV/VIS quantification in HPLC, whether in quality control, regulated environments or analytical research.