AS 100 T automatic sampler

ASI 100 T – Flexibility, precision and reliability for automated HPLC injection

Designed to meet the high demands of analytical laboratories, the ASI 100 T from Thermo Fisher Scientific is a sophisticated automatic sampler for automating sample injection into high-performance liquid chromatography (HPLC) systems. This model, which features integrated high-performance thermal control, is particularly suitable for analyses requiring strict thermal stability of samples, thanks to its Peltier control system capable of maintaining temperatures between 4 and 45 °C.

The ASI 100 T uses in-line split loop injection technology, integrating the needle into the sampling circuit. This configuration significantly reduces the sample volume required while improving injection accuracy and reproducibility. A minimum volume of only 1 µL can be injected, with a standard volume of 5 µL required for a complete injection. In addition, this method minimises cross-contamination through effective internal washing, reducing sample carryover to less than 0.1%.

The system features a modular carousel divided into three 120° sectors, each of which can be configured for different vial types and sizes, including 0.25 mL to 4 mL formats. This allows multiple vial types to be mixed on the same carousel, while the device automatically identifies the formats installed. This makes the ASI 100 T extremely flexible for various analysis protocols.

In terms of programming, the user can configure sample sequences either via the device's intuitive control panel or via the CHROMELEON™ software, which offers a more advanced interface for injection management, dilution automation, reagent addition and complex batch analysis. In addition, a sequence in progress can be interrupted to insert a priority sample using the "Quick Sample" function.

The device is robust and designed for easy maintenance. Critical components such as the syringe and sampling loop are easily accessible for replacement. The system also records usage data (number of injections, valve movements, wear and tear on parts), facilitating preventive maintenance.

Equipped with numerous safety features, such as automatic vial detection, electronic needle protection and leak monitoring, the ASI 100 T guarantees safe, autonomous operation even in demanding analytical environments. This device is a complete and reliable solution for modern laboratories seeking to maximise the productivity and accuracy of their chromatographic analyses.

• Display: LCD screen, control keys, LEDs for sample position and retention time
• Materials in contact: 316 stainless steel (1.4571), PCTFE, PTFE, PEEK, Vespel®
• Temperature range (ASI 100 T): 4 °C to 45 °C (max. cooling -18 °C below ambient)
• Operating pressure: up to 340 bar (4930 psi)
• Operating conditions: Ambient temperature 10–35 °C, relative humidity 40–85%
• Interfaces: 2x RS-232, MSV, sample position, digital inputs/outputs
• Power supply: 115 V / 60 Hz or 230 V / 50 Hz, max. power consumption 126 VA
• Dimensions (H x W x D): 36 x 40 x 45 cm
• Weight: 17 kg
• Load capacity: Up to 25 kg when stacked
• Sample capacity:
  • 63 x 4 mL vials
  • 117 x 1.8 mL vials
  • 192 x 1.2 mL vials
• Injection volume:
  • Standard: 1 to 250 µL (250 µL syringe)
  • Extension: up to 2500 µL (2500 µL syringe, model ASI-100P)
• Volume increment: 0.1 µL
• Reproducibility:
  • RSD < 0.3% at 5 µL
  • Typical RSD < 1% at 1 µL
  • RSD < 0.3% at 100 µL (2500 µL syringe)
• Carryover (cross-contamination): < 0.1%
• Number of repeat injections: Up to 9
• Safety features:
  • Automatic vial and segment detection
  • Electronic needle protection
  • Leak detection
  • Internal monitoring of mechanical components