Simplified cluster generation with Illumina's cBot
Illumina's cBot plays a central role in the next-generation sequencing workflow, replacing traditional, time-consuming methods with a compact, high-performance automated solution. This system creates clonal clusters from single-stranded DNA templates through isothermal bridge amplification performed directly in a sealed flow cell, avoiding frequent handling and the risk of contamination associated with emulsion PCR methods.
The cBot can prepare sequencing templates from more than 96 samples in just 4 to 5 hours, compared to more than 24 hours for older techniques. Manual intervention time is less than 10 minutes, freeing up time for other laboratory activities. Its intuitive software, combined with an 8-inch touchscreen, provides step-by-step guided navigation and real-time progress monitoring. It is also possible to monitor and manage multiple cBots remotely via a browser or smartphone, with notifications sent when the cycle is complete or intervention is required.
The system is based on TruSeq reagents pre-packaged in 96-well plates, eliminating preparation errors, complex storage requirements and potential sources of contamination. The integration of an automatic piercing manifold and uniform thermal connections contributes to optimal cluster density without the need for additional external equipment. Cluster generation results in over 200 million aggregates per flow cell, each containing approximately 1,000 molecules, ready for sequencing with high accuracy.
The process relies on immobilising DNA on a functionalised surface, followed by clonal amplification, 3' end blocking, and sequencing primer hybridisation. Thanks to this rigorous methodology, cBot guarantees efficient, reproducible and reliable production of DNA templates for Illumina sequencing platforms.
