CFX384 Touch: high-capacity real-time PCR system in a 384-well format for accurate and reproducible results
The CFX384 Touch Real-Time PCR System from Bio-Rad is a tool designed for researchers who demand performance, speed and flexibility. This system enables real-time PCR analysis on 384 wells with remarkable efficiency, even at low reaction volumes (down to 3 µl). It features an integrated touchscreen that simplifies cycle initiation without the need for a constant computer connection, while remaining compatible with networked operation of multiple instruments.
At the heart of the CFX384 Touch is proven thermal technology from the C1000 Touch thermal cycler, ensuring exceptional temperature uniformity under all conditions. Each well reaches the target temperature with an accuracy of ±0.2°C at 90°C and uniformity of less than ±0.4°C between wells. This thermal control is complemented by a thermal gradient function of up to 24°C, enabling rapid optimisation of hybridisation temperatures.
Optically, filtered LED detection and photodiodes ensure high sensitivity andno crosstalk between channels. The system can detect up to four distinct fluorophores per well without any channel having to serve as a passive reference, maximising information recovery in multiplexed analyses. It is also compatible with FRET experiments, thanks to a dedicated channel for single-colour analysis.
CFX Maestro software enables statistical analysis (t-tests, ANOVA, etc.), data visualisation in various graphical formats, and export of results suitable for publication. It supports secure data management with electronic signatures, file encryption and an audit trail compliant with FDA 21 CFR Part 11 in its secure version.
Integration with the CFX Automation System II also allows plate processing to be automated and significantly increases analysis throughput, a real advantage for laboratories involved in genotyping, gene expression or mutation analysis campaigns.
The CFX384 Touch is a comprehensive, reliable and accurate system, ideal for environments requiring high throughput, rigorous traceability and maximum adaptability to experimental requirements.