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CFX384 Touch Thermal Cycler

Brand: Bio-Rad

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The Bio-Rad CFX384 Touch system is a 384-well real-time PCR thermocycler designed to deliver reliable and reproducible performance in high-throughput laboratories. Equipped with an intuitive touchscreen and LED optical technology, it enables multiplex detection of up to four targets with remarkable accuracy. This compact system, capable of operating autonomously or in a network with multiple devices, ensures optimal thermal uniformity and smooth data analysis via the PC- and Mac-compatible CFX Maestro software.

Description

CFX384 Touch: high-capacity real-time PCR system in a 384-well format for accurate and reproducible results

The CFX384 Touch Real-Time PCR System from Bio-Rad is a tool designed for researchers who demand performance, speed and flexibility. This system enables real-time PCR analysis on 384 wells with remarkable efficiency, even at low reaction volumes (down to 3 µl). It features an integrated touchscreen that simplifies cycle initiation without the need for a constant computer connection, while remaining compatible with networked operation of multiple instruments.

At the heart of the CFX384 Touch is proven thermal technology from the C1000 Touch thermal cycler, ensuring exceptional temperature uniformity under all conditions. Each well reaches the target temperature with an accuracy of ±0.2°C at 90°C and uniformity of less than ±0.4°C between wells. This thermal control is complemented by a thermal gradient function of up to 24°C, enabling rapid optimisation of hybridisation temperatures.

Optically, filtered LED detection and photodiodes ensure high sensitivity andno crosstalk between channels. The system can detect up to four distinct fluorophores per well without any channel having to serve as a passive reference, maximising information recovery in multiplexed analyses. It is also compatible with FRET experiments, thanks to a dedicated channel for single-colour analysis.

CFX Maestro software enables statistical analysis (t-tests, ANOVA, etc.), data visualisation in various graphical formats, and export of results suitable for publication. It supports secure data management with electronic signatures, file encryption and an audit trail compliant with FDA 21 CFR Part 11 in its secure version.

Integration with the CFX Automation System II also allows plate processing to be automated and significantly increases analysis throughput, a real advantage for laboratories involved in genotyping, gene expression or mutation analysis campaigns.

The CFX384 Touch is a comprehensive, reliable and accurate system, ideal for environments requiring high throughput, rigorous traceability and maximum adaptability to experimental requirements.

Features

  • Integrated touchscreen for stand-alone use
  • 384-well format for high-throughput analysis
  • Multiplex function for up to 4 targets
  • Temperature optimisation via thermal gradient
  • Rapid and precise thermal uniformity
  • Compatible with automation systems
  • Safety features (Maestro Security version)

Technical Details

Thermal cycler:

  • Frame: G100 Touch
  • Maximum ramping speed: 5 °C/sec
  • Average speed: 2 °C/sec
  • Heating/cooling method: Peltier
  • Temperature range: 0–100 °C
  • Accuracy: ±0.2 °C at 90 °C
  • Uniformity: ±0.4 °C within 10 sec at 90 °C
  • Gradient function: 30–100 °C with a programmable deviation of 1 to 24 °C

Optical detection:

  • Filtered LEDs (x5) and filtered photodiodes (x5)
  • Detection range: 450–690 nm
  • Sensitivity: detection of a single copy of DNA
  • Dynamic range: 10 orders of magnitude
  • Scan time: <20 sec (all channels), 8 sec (single-channel fast scan)

CFX Maestro software:

  • Operating systems: Windows 7/10 (64-bit), macOS 10.14 (analysis only)
  • Analysis: PCR quantification, melting curve, gene expression (ΔΔCq or ΔCq)
  • Graphical tools: bars, box plots, clusters, volcano plots, etc.
  • Export: .jpg, .png, .bmp, Word, Excel, PowerPoint, PDF

Compatible Accessories

Additional modules:

  • GFX384 Optical Reaction Module
  • CFX Automation System II

Software:

  • Precision Melt Analysis Software
  • qbase+ Software

Reagents and consumables:

  • iScript cDNA Synthesis Kit, SYBR® Green Supermix, Probes Supermix
  • Hard-Shell 384-Well Plates
  • Microseal 'B' and 'F' for PCR

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Logistics

Remma and its partners provide you with serviced and configured equipment tailored to your needs. Receive your product at your facility with standard or express delivery, and an installation option, all through a single point of contact.

Warranty

Our 12/24-month warranty ensures the safety and reliability of your medical equipment throughout its lifespan.

Flexible Financing

Settle your medical equipment in up to 36 installments with our financing service or pay after delivery with Remma Facility.