Optimising Selectivity and Productivity in Proteomics with the FAIMS Pro Interface
The Thermo Scientific FAIMS Pro interface represents a major advance in proteomics, offering increased selectivity and sensitivity for protein identification and quantification. Designed to integrate seamlessly with Orbitrap Fusion Lumos Tribrid and Orbitrap Fusion Tribrid mass spectrometers , this interface uses differential ion mobility technology to improve ion separation, thereby reducing chemical noise and matrix interference.
One of the main advantages of the FAIMS Pro interface is its ability to reduce sample preparation steps , which are often time-consuming and complex. By performing online gas phase fractionation, it increases proteome coverage without additional work, while preserving the sample. This translates into a significant increase in productivity and data quality for every user, from disease biomarker discoveries to the identification of new therapeutic targets.
The FAIMS Pro interface is also designed to be easy to install, use and maintain. Its tool-free assembly and predefined workflows for data acquisition and processing ensure ultimate usability and high data quality for all users, regardless of their level of expertise. Furthermore, its compatibility with low-flow chromatographic separations and direct infusion experiments makes it a versatile tool for a wide range of proteomics applications.
In terms of performance, the FAIMS Pro interface offers an optimised cylindrical geometry that increases ion transmission to the mass spectrometer with short residence times, allowing multiple compensation voltage (CV) settings per data acquisition method. This allows repeated acquisition of multiple CVs, increasing the number and type of precursors detected and sequenced, resulting in higher identification rates.
The Thermo Scientific FAIMS Pro interface is a powerful tool for any proteomics laboratory seeking to improve the selectivity, sensitivity, and productivity of their analyses. Its ease of use, compatibility with existing systems, and superior performance make it a valuable investment for any proteomics research facility.
