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Fixed-angle rotor JA-25.50


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The Beckman Coulter JA-25.50 fixed-angle rotor is designed for use with Avanti® J and J2 series centrifuges. Made from anodized aluminum, it holds up to eight 50 mL tubes and reaches speeds of up to 25,000 rpm, generating a relative centrifugal force (RCF) of up to 75,600 × g. Featuring a single or dual-locking lid and a patented fluid containment annulus, the JA-25.50 ensures high performance and optimal sample safety. It is ideal for separating cells, viral particles, or biological components from large sample volumes with precision, safety, and reliability. 

Description

JA-25.50: A high-performance rotor for demanding applications

The JA-25.50 rotor is a key component of Beckman Coulter’s high-capacity centrifuges in the Avanti® J and J2 series. Engineered for performance and safety, this rotor delivers high-speed separations with precision while maintaining structural integrity, even under demanding lab conditions. It is especially suitable for processing large volumes of cells, tissue homogenates, or virus particles.

Constructed from anodized aluminum, the JA-25.50 combines durability with lightweight design. Its 34-degree fixed-angle configuration accommodates eight 50 mL tubes, providing a total capacity of 400 mL. Its patented fluid containment annulus captures any potential spills, effectively keeping fluids contained within the rotor, even if all eight tubes were to leak simultaneously. This feature greatly reduces the risk of chamber contamination and ensures biosafety compliance.

It has been independently validated for microbiological containment under standard operating conditions. Integrated finger grips around each cavity provide added convenience for loading and unloading tubes, enhancing usability.

This rotor is compatible with a wide variety of labware, including polycarbonate, polyallomer, and polypropylene tubes with or without caps. Partial and full fills are supported, provided that opposing cavities are balanced with equal volume and density. For smaller volumes, specific adapters are available.

The rotor is easy to maintain and can be autoclaved at 121°C. With proper cleaning, lubrication, and storage as recommended, the JA-25.50 ensures long-term reliability, even in high-throughput laboratory environments.

Features

  • Durable and lightweight anodized aluminum rotor body
  • 34° fixed-angle design for efficient sedimentation
  • Compatible with Beckman Coulter Avanti® J and J2 series centrifuges
  • Secure single or dual-locking lid system
  • Patented fluid containment annulus to prevent chamber contamination
  • Finger grips for easy handling and tube loading/unloading
  • Independently validated for microbiological aerosol containment
  • Supports full or partial tube filling with proper balancing
  • Compatible with a wide range of tube types and materials
  • Autoclavable at 121°C for up to 1 hour
  • Easy cleaning and maintenance using Beckman-recommended products


Technical Details

  • Maximum speed: 25,000 rpm
  • Maximum RCF (at rmax 108 mm): 75,600 × g
  • Average RCF (rav 73.2 mm): 51,200 × g
  • Minimum RCF (rmin 38.5 mm): 27,000 × g
  • Capacity: 8 × 50 mL tubes (400 mL total)
  • Fixed angle: 34°
  • Material: anodized aluminum
  • Empty rotor weight: 8.1 kg
  • Weight with maximum load: 8.7 kg
  • Critical speed range: 600 to 800 rpm
  • Acceleration time (to 25,000 rpm): 3.5 min (Avanti J), 1.75 min (J2)
  • Deceleration time: 1.75 min (Avanti J), 2.5 min (J2)
  • Rotor code for J2 centrifuges: 25.5
  • k factor: 418
  • Operating temperature range: 4–24°C (depending on centrifuge model)

Compatible Accessories

  • Large lid O-ring (ref. 870612)
  • Small lid O-ring (ref. 011757)
  • Dual-locking lid (ref. 363023)
  • Single-locking lid (ref. 363053)
  • Rotor cleaning kit (ref. 339558)
  • Rotor removal tool (ref. 346965)
  • Spinkote lubricant (ref. 306812)
  • Beckman cleaning solution 555 (ref. 339555)
  • Compatible bottles and tubes (polycarbonate, polyallomer, polypropylene)
  • Adapters for small volumes (ref. 361703, 361705, etc.)

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