Optimized Multicolor Analysis with the BD LSR II Flow Cytometer
The BD LSR II from BD Biosciences is a flow cytometer designed to meet the diverse needs of research laboratories seeking reliable, accurate, and scalable cellular analysis. This modular system supports up to four fixed and aligned lasers: a UV laser (355 nm), a violet laser (405 nm), a blue laser (488 nm), and a red laser (633 nm). This architecture enables efficient fluorochrome distribution to minimize spectral overlap and enhance detection sensitivity.
A key strength of the BD LSR II lies in its optical system. It features patented octagon and trigon optical arrays that maximize light collection and boost detection performance. Each laser beam is directed to a series of photomultiplier tubes (PMTs) via fiber optics, ensuring consistent and sensitive signal detection—even in complex multicolor experiments. Interchangeable bandpass filters and dichroic mirrors allow for quick adjustment of detection parameters without compromising signal quality.
Data acquisition is handled by a high-performance digital system operating at 10 MHz (10 million samples per second), enabling processing of up to 20,000 events per second. This digital approach improves linearity, fluorescence compensation, and rare population analysis. The BD FACSDiva™ software simplifies experimental design, instrument control, and data analysis with reusable templates, automated compensation, and biexponential display options.
In terms of ergonomics and fluidics, the BD LSR II offers multiple operational modes (RUN, STANDBY, PRIME), a regulated pressure-based fluidics system, and a quartz flow cell optimized for light transmission. It can be paired with a High Throughput Sampler (HTS) for automated processing of 96- or 384-well plates.
Engineered for high-content flow cytometry, the BD LSR II enables complex analyses such as calcium flux measurement, stem cell identification with UV dyes, and antigen-specific T-cell phenotyping using multicolor panels. With its compact benchtop design, flexible configuration, and advanced digital performance, the BD LSR II stands as a reference instrument in biomedical research.
