Applied Biosystems GeneChip Fluidics Station 450: Optimisation of washing and staining protocols
The GeneChip Fluidics Station 450 is an essential platform for laboratories using GeneChip technology. It fully automates the critical steps of washing and staining probe cartridges, which are essential for preparing samples for scanner analysis. Each module of the instrument can be programmed independently, allowing different protocols to be run in parallel while using the same wash reagents. This flexibility contributes to better workflow organisation and increased efficiency in experiment management.
Thanks to its ergonomic design, the system allows for simple and secure loading of cartridges, reducing the risk of leaks and technical errors. The transition from angular to linear movement during cartridge insertion improves the reliability of fluid connections and reduces the risk of failure.
Reliability and experimental quality control
The reproducibility of experimental results is at the heart of the GeneChip Fluidics Station 450. The instrument is equipped with fluid detection sensors that measure flow resistance, ensuring accurate identification of the presence or absence of solution. This system minimises handling errors and ensures that the protocol progresses smoothly without interruption. In the event of an anomaly, clear error codes are displayed on the LCD screen, allowing the user to quickly identify the cause of the problem and apply an immediate solution.
Centralised management via the GeneChip Operating Software (GCOS) provides a user-friendly interface for defining, monitoring and customising protocols. The operator can adapt the washing, temperature and staining cycle parameters to the specific requirements of the experiment. The software also allows up to eight fluidic stations to be connected to a single workstation, making the system scalable for high-throughput laboratories.
A robust tool for genomics research laboratories
The GeneChip Fluidics Station 450 is built to integrate into a demanding research environment. Its robustness, combined with an accessible maintenance programme (weekly cleaning protocols with deionised water and sodium hypochlorite), ensures a long service life and consistent performance. Users can replace certain modules and components, such as the peristaltic tubing, themselves, reducing downtime and maintenance costs.
The device is part of Applied Biosystems' portfolio of solutions for genomic research, particularly in combination with GeneChip scanners. Its compatibility with various genotyping and gene expression protocols makes it an indispensable tool for laboratories seeking to standardise and improve the reliability of their analyses. Weighing approximately 36 kg and with dimensions suitable for a bench-top workstation, it is designed for intensive use in a controlled environment, with an operating temperature range of 15 to 30°C.
