Agilent G1321A Fluorescence Detector: Performance, accuracy and sensitivity in liquid chromatography
The Agilent G1321A fluorescence detector is a reference analytical instrument for liquid chromatography applications requiring extremely sensitive detection of fluorescent compounds. It is based on technology that combines a xenon flash lamp, a dual monochromator optical system and a high-performance photomultiplier to provide low-noise detection capability, even at very low concentrations.
At the heart of the G1321A is a sophisticated optical system incorporating a xenon lamp that produces a continuous light spectrum from 200 to 900 nm. This light source is then directed via a holographic concave diffraction grating to the flow cell, allowing precise excitation of the analytes. The fluorescence emission is then captured at 90° via a second monochromator, filtered, amplified and analysed by a photomultiplier tube. This optimised optical path ensures maximum reduction of stray light and high detection efficiency.
The detector allows analysis in single or dual wavelength mode (λEx / λEm) and programming of up to four wavelengths for increased flexibility. It also features an automatic correction system based on a reference photodiode that compensates for fluctuations in lamp intensity, ensuring high long-term stability of results. Excitation and emission spectra can also be acquired, facilitating method development and compound identification.
Robust and compact, the G1321A is designed to integrate into Agilent LC 1200 systems and withstand demanding laboratory environments. It can be used with a range of flow cells, including bio-inert or low-volume versions, suitable for sensitive samples or low flow rates.
The G1321A fluorescence detector is ideal for analyses in the pharmaceutical, environmental, food and biomedical research fields. It offers high-level analytical performance while simplifying installation, maintenance and operation thanks to its integrated GLP features and built-in diagnostic tools.
