Amersham IPGphor – Isoelectric focusing system for 2D electrophoresis
The Amersham IPGphor system is a specialised instrument for performing first-dimensional isoelectric focusing (IEF) in two-dimensional electrophoresis. It uses Immobiline DryStrip strips, which incorporate an immobilised pH gradient, to separate proteins according to their precise isoelectric point. Thanks to a design that integrates both rehydration and focusing in a single support, the IPGphor guarantees simplified handling and optimal reproducibility. The device has different types of strip holders – fixed or cup-loading – to adapt to various protocols and strip lengths ranging from 7 to 24 cm. The central unit includes a continuous high-voltage power supply, thermal control via Peltier thermoelectric modules and a clear interface with a membrane keyboard and LCD screen.
Performance and flexibility for laboratory applications
The IPGphor can store up to ten customised protocols, each with up to nine programmable steps. Adjustable parameters include rehydration time and temperature, current limit per strip, voltage at each step, step duration and voltage variation mode (stepping or gradient). This flexibility allows the focus to be precisely tailored to specific needs, whether for analysing complex samples or optimising the resolution of targeted proteins. The ability to accommodate up to twelve fixed-length media or nine cup-loading media simultaneously maximises laboratory efficiency, particularly during comparative analyses or high-throughput projects. The device accepts a wide range of strips with linear or non-linear pH gradients, covering many applications in proteomics, molecular biology and biomedical research.
Safety, ergonomics and workflow integration
Designed for intensive use in research environments, the IPGphor incorporates several safety features, including automatic high-voltage shut-off when the cover is opened. Gold-plated copper electrode areas ensure excellent conductivity and extended durability. The strip supports are made of aluminium oxide ceramic treated to limit protein adsorption, thus ensuring separation quality and ease of maintenance. Cleaning is simple thanks to the specific solution provided, which preserves the surface properties of the supports.
Ergonomics are enhanced by a clear user interface, allowing intuitive navigation between settings, and by the ability to modify certain values during operation. The IPGphor also has an RS232C serial port for connection to a computer or printer, facilitating documentation and analysis tracking. Thanks to its combination of precision, versatility and safety, the IPGphor system is an essential tool for preparing gels for two-dimensional electrophoresis and obtaining high-quality protein profiles.
