Laser Microdissection System LMD7000 - Good 2013

Leica LMD7000 Laser Microdissection System: Precision Sample Preparation for Advanced Research

The LMD7000 is a high-precision laser microdissection system designed to meet the demands of molecular research. It allows for the preparation of exceptionally precise samples, ideal for applications in fields such as genomics, transcriptomics, proteomics, and other complex biological analyses. Using a UV laser, this system enables the dissection of tissues and the isolation of cells in a highly targeted manner, ensuring the quality and integrity of samples for molecular biology research, next-generation sequencing (NGS), biochip analysis, and applications involving living cells. The LMD7000 is particularly suited for research in oncology, neuroscience, plant biology, and many other scientific domains.

Laser microdissection (LMD) has become an essential technology in various biomedical and biological research fields. The LMD7000 system uses high-precision laser technology to dissect tissues, allowing the isolation and manipulation of individual cells or cell populations from heterogeneous samples. This ability to separate complex samples precisely is crucial in research fields such as genetic sequencing, proteomics, and cell biology.

This system ensures meticulous separation of tissues or cells without affecting other parts of the sample, leading to homogeneous samples and preventing contamination. The patented gravity-based collection system used by the LMD7000 ensures non-invasive, contact-free, and contamination-free collection, which is vital when working with fragile samples such as living cells, embryos, or rare and complex tissues like bone, teeth, or plant tissues.

The LMD7000 is distinguished by its high-power UV laser, which offers great flexibility to handle various tissue types and samples. The laser beam allows for extremely fine dissection with micrometer precision, enabling researchers to cut tissues or cells precisely without damaging adjacent samples. This process is flexibly controlled through an intuitive software interface, allowing users to adjust the laser's power, pulse frequency, speed, and focal point in real time to suit the specific needs of the sample and the analysis.

The LMD7000 also stands out for its ability to perform dissection on living cells. One of the key aspects of this system is its flexibility for real-time cell manipulation applications. Using sterile chambers or membrane rings, the system allows for the dissection and manipulation of living cells without disturbance. After dissection, the cells can be directly transferred into culture media for reculture or into collectors for further analysis.

Additionally, the system supports time-lapse tracking through an integrated imaging function, which is particularly useful for research on living cells and the dynamic monitoring of biological processes. An integrated climate chamber ensures a stable, controlled environment, thus maintaining optimal conditions for dissection and manipulation of living cells.

The LMD7000 is designed to be both easy to use and highly flexible. Its LMD software, based on simplified workflows, allows researchers to take full control of all system parameters in an intuitive manner. The system also offers additional modules, such as automatic pattern recognition (APR) and automated image capture, enabling comprehensive documentation and tracking of samples.

• Laser Wavelength: 349 nm
• Pulse Frequency: 10–5,000 Hz
• Pulse Length: < 4 ns
• Max Pulse Energy: 120 µJ
• Laser Settings: Adjustable laser power, aperture, speed, frequency (LMD7000 specific), and focus
• Objectives: Dedicated LMD objectives (5x, 6.3x, 10x, 20x, 40x, 63x, 150x)
• Fluorescence Integration: Fully integrated fluorescence with specially designed filter cubes
• Microscope Type: Fully automated high-end upright research microscope
• Software: Easy-to-use LMD software with automated pattern recognition, image capture, and documentation modules