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Mithras LB 940 microplate reader

The Mithras LB 940 from Berthold Technologies is a multimode microplate reader designed to meet the most complex requirements of modern laboratories. It enables the analysis of multiple signal types – luminescence, fluorescence, absorbance, FRET, BRET, AlphaScreen™ and polarised fluorescence – with exceptional sensitivity. Thanks to its modular design, integrated reagent injectors and powerful software, it adapts to all types of tests, from fundamental research to high-throughput screening. Its flexibility, precision and compatibility with all plate formats make it a benchmark instrument.



Description

Mithras LB 940 – The multimode microplate reader that exceeds the expectations of demanding laboratories

The Mithras LB 940 is much more than just a microplate reader: it is a central tool for laboratories seeking to combine flexibility, high performance and analytical precision. With dedicated optical paths for each detection technology, it delivers performance equivalent to specialised devices while centralising the detection of luminescence, fluorescence (top and bottom reading), polarised fluorescence, absorbance, FRET, BRET and AlphaScreen™.

Each measurement technology is optimised by an independent optical system, ensuring optimal sensitivity and a wide dynamic range. Integrated variable-volume injectors enable precise injection during measurement, ideal for kinetic assays or flash reactions. The Mithras LB 940 also offers an automatic plate height adjustment system, an essential feature for automated processing of plates with 6 to 1536 wells. Its ergonomic design and compact footprint make it easy to integrate into any laboratory environment, whether in academic research, the pharmaceutical industry or biotechnology.

The software flexibility allows complex measurement sequences to be automated with excitation/emission filter management in a matter of milliseconds, which is crucial for ratiometric measurements, fast kinetic assays or multi-wavelength tests. The integrated shaking system, optional temperature control and robotic integration further enhance the versatility of the device. The intuitive MikroWin software interface allows even novice users to get started quickly, while offering advanced features such as kinetic analysis, mathematical adjustments and compatibility with the most stringent regulatory requirements.

Whether for studying cell viability, protein interactions, G protein-coupled receptors or quantifying molecular targets, the Mithras LB 940 delivers reproducible results with detection sensitivity in the attomole range. It is particularly suited to the fields of pharmacology, cell biology, molecular genetics and immunological testing. Thanks to its modular options and scalable architecture, it provides long-term support to laboratories in their most innovative research.

Its compatibility with safety standards (21 CFR Part 11), its integration into robotic chains, its plate identification system and its user access control make it an all-in-one solution that is perfectly aligned with today's needs for reliability, traceability and productivity. The Mithras LB 940 is truly a strategic investment for any organisation seeking performance, precision and adaptability in microplate analysis.

Features

  • Dedicated optical paths for each technology
  • Fast emission/excitation filter switching (130 ms)
  • Reading of plates from 6 to 1536 wells, Petri, Terasaki
  • 3-mode shaking system (variable amplitude and speed)
  • Automatic plate height adjustment (8 to 22.2 mm)
  • Temperature control from +5 °C to 37 °C
  • Integral, kinetic and repeated measurements with programmable delay
  • Reagent injection during kinetic measurement
  • 20 or 100 plate stackers (Stacker20 / Stacker100)
  • Robotic integration with optional barcode reader
  • Data security: electronic signatures, audit trail
  • Data export: ASCII, Excel® formats

Technical Details

  • Dimensions: 472 × 494 × 374 mm
  • Weight: 52 to 54 kg depending on configuration
  • Power supply: 110–230 V, 50/60 Hz, 240 VA
  • Operating temperature: 15° to 35°C
  • Storage temperature: 0° to 40°C
  • Humidity: 10–85% non-condensing
  • Detection technologies:
    • Luminescence (flash, glow, multi-wavelength)
    • Fluorescence (top and bottom reading)
    • Polarised fluorescence
    • Absorbance
    • FRET, BRET, BRET2
    • AlphaScreen™
  • Detection limits:
    • < 20 amol ATP (luminescence)
    • < 20 fmol FITC (fluorescence)
    • AlphaScreen™: < 12.5 ng standard beads
  • Dynamic range: > 6 orders of magnitude
  • Low cross-interference: 5 × 10⁻⁶
  • Injectors: up to 4, volume 10–100 µl
  • Software: MikroWin 2000, Lite, Advanced I/II versions
  • Interface: RS232, compatible with Win XP / 2000 / NT

Compatible Accessories

  • Injection modules 1 to 4 (10–100 µl)
  • Waste pump for injectors
  • FRET, BRET, AlphaScreen™, Absorbance, Polarised Fluorescence modules
  • Cooling unit for photomultiplier (PMT)
  • Bottom-reading modules
  • Plate height adjustment
  • Automatic stackers: 20 or 100 plates
  • MikroWin 2000 Lite, Advanced I, Advanced II software
  • Robotic integration with specific modules

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Logistics

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Warranty

Our 12/24-month warranty ensures the safety and reliability of your medical equipment throughout its lifespan.

Flexible Financing

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