Mastercycler ep gradient Thermocycler - Perfect Condition

Eppendorf Mastercycler ep gradient: precision thermal cycler for PCR

The Eppendorf Mastercycler ep gradient is a thermal cycler for the most demanding PCR applications. Designed for maximum flexibility, it features precise temperature control, an intuitive user interface and programming capabilities. The system is ideal for molecular biology research laboratories where reproducibility and performance are essential.

This model allows temperature gradients of up to 20°C or 24°C, depending on the version, facilitating the optimisation of primer hybridisation temperatures. Thanks to this feature, several experimental conditions can be tested in a single PCR cycle, significantly speeding up protocol development.

The Mastercycler ep gradient is equipped with ESP (Electronic Sample Protection) technology, an electronic heating lid that applies optimally controlled pressure to PCR tubes or plates. This feature significantly reduces sample evaporation, ensures excellent thermal uniformity and protects samples during temperature ramps.

The device is compatible with different formats: 0.2 ml PCR tubes or 96-well PCR plates. It also offers significant modularity thanks to the possibility of connecting several thermocyclers to a single control panel. Up to five Mastercycler ep devices can be managed simultaneously, and a complete network of 30 units can be administered via the CycleManager software.

The control screen allows smooth navigation through the user and programme tree, facilitating the creation, editing and execution of protocols. The system also supports MultiMediaCard™ cards for data storage and protocol backup.

Its graphical programming system makes PCR cycle design very intuitive. Each step can be customised with functions such as temperature ramps, time increments, gradients or constant temperature maintenance. The user can also simulate the thermal behaviour of an older Mastercycler model to transfer existing protocols without loss.

Thanks to its rapid heating capabilities (up to 6 °C/s for certain versions) and exemplary thermal homogeneity (±0.3 °C at 35 °C), the Mastercycler ep gradient ensures reliable and reproducible results. It also features integrated safety functions such as emergency lid unlocking, automatic shutdown at low temperatures and PIN code management for secure user access.

The Mastercycler ep gradient is a versatile, scalable and accurate device designed to support researchers in all stages of PCR, from optimisation to routine use.

• Sample capacity: 96 x 0.2 ml PCR tubes or 1 PCR plate with 96 wells (gradient versions)
• Block temperature range: 4 °C to 99 °C
• Gradient range: up to 20 °C (gradient), 24 °C (gradient S)
• Gradient range: 30 °C to 99 °C
• Heating lid temperature: 37 °C to 110 °C
• Temperature uniformity at 35 °C: ± 0.3 °C
• Temperature uniformity at 90 °C: ± 0.4 °C
• Control accuracy: ± 0.2 °C
• Heating speed: up to 6 °C/s (depending on model)
• Dimensions (W x D x H): 26 x 41 x 30.5 cm
• Weight: 17 kg
• Power supply: 100–130 V or 200–240 V, 50–60 Hz
• Power consumption: 800 W
• Ambient conditions: 15 °C to 35 °C; relative humidity 70%