Microplate reader SpectraMax Paradigm

SpectraMax® Paradigm®: Multi-Mode Microplate Reader for Precise and Flexible Analysis

The SpectraMax® Paradigm® is a multi-mode microplate reader designed to meet the demands of modern research laboratories. This versatile device can perform a variety of measurements, including absorbance, fluorescence, luminescence, and polarized fluorescence, while offering exceptional flexibility thanks to its modular detector cartridges.

The SpectraMax® Paradigm® is distinguished by its ability to automatically optimize the Z-height, improving the dynamic range and sensitivity of assays. This feature allows the lens to be adjusted to accommodate small volumes or varying well geometries, ensuring accurate and reliable results. In addition, the detector cartridge system allows new reading modes to be added in minutes without requiring a service visit, providing adaptability for future applications.

With SoftMax® Pro software, users can perform multiple calculations on a single dataset, compare multiple experiments side-by-side, and adjust reduction parameters while collecting data in real time. This software integration enables in-depth data analysis, including EC50 and Z-factor analysis to assess assay quality, as well as visualization of results with over 21 regression curve options.

The SpectraMax® Paradigm® is compatible with a wide range of plate types, from 6 to 1536 wells, and offers temperature control from ambient +4°C to 45°C. It also features linear and orbital shaking capabilities, as well as automation capabilities for easy integration into laboratory workflows.

The SpectraMax® Paradigm® is an indispensable tool for laboratories requiring precise and flexible analysis, offering a comprehensive range of features to meet the evolving needs of scientific research.

• Dimensions: Height 45 cm x Width 39.2 cm x Depth 63 cm
• Reading Modes: Absorbance, Fluorescence (top/bottom reading), Luminescence (top/bottom reading), Time-Resolved Fluorescence (top/bottom reading), Fluorescence Polarization (top/bottom reading), HTRF (top/bottom reading), AlphaScreen (top/bottom reading)
• Wavelength Range: Abs: 230 - 1000 nm, FL Ex: 360 - 790 nm, FL Em: 400 - 850 nm, Lumi: 360 - 850 nm, TRF Ex: 360 - 790 nm, TRF Em: 400 - 850 nm
• Wavelength Selection: Cartridge filters and monochromator adjustable in 1 nm increments
• Accuracy Photometric/Absorbance Repeatability: Cartridge: ±(1% + 0.005) OD at 2 OD
• Optimized Top Read Sensitivity: FL Cartridge: 0.1 fmol/well in 75 μL Fluorescein 384-well, 0.03 fmol/well in 8 μL Fluorescein 1536-well, Lumi Cartridge: 0.3 fmol/well in 200 μL ATP-Glow 96-well (1.5 pM), 0.2 fmol/well in 50 μL ATP-Glow 384-well (4 pM), TRF Cartridge: 3 amol/well in 100 μL Europium (0.03 pM) 384-well, 1 amol/well in 8 μL Europium (0.125 pM) 1535-well, FP Cartridge: 3 mP 75 μl fluorescein 384-well, 6 mP 8 μl fluorescein 1536-well, AlphaScreen Cartridge: <100 amol/well 384-well
• Light Sources: Xenon Flash Lamp, Ultra High Power LED
• Detectors: Silicon Photodiode, Photomultiplier Tube
• Read Types: Endpoint, Kinetic, Spectrum Scan, Well Scan
• Read Time: Abs: 19 seconds, FL: 13 seconds, Lumi: 11 seconds, TRF: 12 seconds, FP: 17 seconds, AS: 22 seconds
• Plate Types: 6- to 1536-well plates
• Temperature Control: Ambient +4°C to 45°C