Illumina MiniSeq: a compact, reliable and accessible NGS sequencing solution
Illumina's MiniSeq is a benchtop NGS sequencing system that allows researchers to harness the power of Illumina technology in an easy-to-use format. It is designed to give laboratories complete autonomy, eliminating the need to wait for sample pools to launch a run on high-throughput equipment. This autonomy is made possible by its ability to support targeted applications with comprehensive gene coverage, while offering fast turnaround times and simplified data management.
With the MiniSeq, users can start sequencing in less than five minutes after loading the library. The system integrates clonal amplification, sequencing and data analysis, without the need for additional equipment. Results are available in less than 24 hours for most applications. The embedded Local Run Manager software allows users to manage sequences, monitor runs and perform analyses directly on the device or via the cloud with BaseSpace Sequence Hub.
The MiniSeq supports a wide variety of applications, from small RNA (miRNA) detection to targeted expression panel sequencing and solid or haematological tumour research. It can be used on both DNA and RNA and is suitable for different types of projects thanks to its medium- and high-throughput kits.
This system is based on Illumina's sequencing-by-synthesis (SBS) chemistry, renowned for its accuracy, particularly in homopolymer-rich regions. Errors are significantly reduced and coverage can be increased without significantly increasing costs. Each run can include between 1 and 96 samples for targeted panels, or up to 384 for expression profiling, with very short analysis times.
Thanks to its compact design, compatibility with Illumina kits, and analysis tools that are accessible even to non-bioinformatics specialists, the MiniSeq is establishing itself as a routine sequencing solution for genomic research.
