MyoStretcher: a high-precision force measurement system for cardiomyocytes
The MyoStretcher from IonOptix represents a major advance in the field of functional myocardial cell research. Designed to measure the force generated by isolated, intact cardiomyocytes, this system is based on OptiForce, a nanoscopic optical force sensor offering sub-nanonewton resolution. This exceptional sensitivity allows the detection of the slightest variations in force, a fundamental requirement in advanced cardiovascular research.
The device has been designed for simple and reliable use, even in laboratories with complex experimental protocols. It includes all the components necessary for stretching and force measurement: high-precision motorised micromanipulators, fixation components and, as an option, a piezoelectric transducer for programmed control of stretching and a fixation kit for glass rods. This configuration ensures perfect adaptation to the specific needs of researchers, whether studying mechanosensitive phenomena or contractile response under load.
The MyoStretcher can operate independently or be integrated as an add-on module to IonOptix systems for measuring intracellular calcium and contractility. This allows for complete synergy between mechanical and calcium dynamics measurements, which is essential for an integrated understanding of cellular functions.
The device allows fine programming of stretching protocols, with the possibility of defining waveforms such as ramps, pulses, trapezoids or sinusoids. This experimental flexibility is ideal for simulating different physiological or pathological conditions. Thanks to the piezo control, the length can be modified with extreme precision, in absolute values or as a percentage, with real-time feedback on the applied force thanks to the "fast-feedback force clamping" mode.
Cell adhesion is ensured by MyoTak, a biocompatible adhesive specially formulated to allow cardiomyocytes to attach to smooth surfaces such as carbon fibres or glass rods without compromising cell integrity. Developed at the University of Maryland, MyoTak guarantees robust attachment without altering cell physiology, enabling reliable and reproducible measurements.
IonOptix's MyoStretcher is designed for scientific precision, modularity and ease of use. It is intended for use in basic research laboratories as well as by pharmacology and translational research teams seeking to accurately quantify the mechanical parameters of cardiac cells. The integration of force data with intracellular calcium data makes it a tool of choice for studying the mechanisms of excitation-contraction coupling, mechanical plasticity and the effects of various cardiac therapies.
