NanoDrop 3300 Fluorometer - Perfect Condition

Sensitive Quantification of Biomolecules in Microvolumes: the Advantage of the NanoDrop 3300 in Medical Laboratories

The NanoDrop 3300 from Thermo Fisher Scientific is a compact fluorometer designed for fluorescent analyses requiring high sensitivity on microvolumes. Specially adapted to the needs of biomedical laboratories, it can quantify nucleic acids or proteins labelled with fluorescent dyes with a mass detection of less than 1 fmol of fluorescein. The system is based on three LED light sources (UV, blue and white) covering a wide excitation spectrum, enabling the detection of a wide range of fluorophores, from Hoechst to Cy5, without the need for optical filters.

With absorbance accuracy of less than 5% CV and a spectrum ranging from 400 to 750 nm, the instrument is perfectly calibrated for applications in molecular medicine, diagnostics, genetics and virology. Its unique retention technology allows measurements to be taken directly on a stainless steel and quartz pedestal, without cuvettes or specific consumables, significantly reducing costs and handling. The volumes required are very low (from 1 µl), thus preserving rare or expensive samples.

The accompanying software, compatible with Windows XP, 2000 and Vista 32-bit, ensures simple and fast reading with immediate display of results. The system is fully powered via USB, with a maximum power consumption of only 2 W. Thanks to its compact size (14 x 20 cm) and light weight of 1.5 kg, the NanoDrop 3300 fits easily into any workspace, even the most confined.

Ideal for high-sensitivity analyses such as those involving PicoGreen®, SybrGreen®, Alexa Fluor® or FRET probes, this device is a reliable tool for medical professionals looking for performance on very low-volume samples.

Thanks to its versatility, it fits just as well into academic laboratory workflows as it does in hospital or pharmaceutical environments requiring accurate quantification of limited samples.

• Instrument type: Fluorospectrometer
• Minimum sample size: 1 µl
• Number of samples: 1 at a time
• Light sources: 3 LEDs (UV, blue, white)
• Excitation wavelengths: UV: 365 nm ±10 nm; Blue: 470 nm ±10 nm; White: 460–650 nm
• Detector: 2048-element linear CCD matrix
• Spectral range: 400–750 nm
• Wavelength accuracy: 1 nm
• Spectral resolution: 8 nm (FWHM at 546 nm)
• Absorbance accuracy: < 5% CV (10 nM fluorescein)
• Fluorescence dynamic range: > 4 logs (fluorescein)
• Detection limit: < 1 fmol (fluorescein)
• Measurement time: 2 to 10 seconds
• Pedestal material: 303 stainless steel and quartz fibre
• Dimensions: 14 x 20 cm
• Weight: 1.5 kg
• Power supply: 5 V DC via USB port
• Power consumption during operation: 2 W
• Standby power consumption: 1 W
• Software compatibility: Windows 2000, XP, Vista (32-bit)
• Compliance: CE and UL/CSA standards