The NextSeq 550: an integrated platform for sequencing and genotyping
The Illumina NextSeq 550 system is designed for research laboratories seeking flexibility, performance and efficiency in their genomic studies. By combining high-throughput sequencing and DNA chip digitisation on a single platform, it meets the diverse needs of both small and large laboratories. This dual capability makes it easy to switch between different types of analysis, including targeted panels, exomes, transcriptomes and expression profiles, without the need for additional equipment.
The platform is designed to adapt to the evolving requirements of laboratories: users can choose between two types of flow cells (medium and high throughput), allowing fine modulation of sequencing capacity depending on the number of samples or the level of coverage required. A single run can process up to 40 samples for gene expression profiles or up to 96 targeted panels. This flexibility saves significant time, with turnaround times ranging from 6.5 hours to less than 30 hours depending on the cycles selected.
The user interface is intuitive, the reagents are ready to use, and the system can be set up in a few simple steps. The NextSeq 550 integrates cluster generation and sequencing, automating the process to reduce human intervention. It is also compatible with all Illumina library preparation solutions, ensuring consistency across workflows.
Data analysis can be performed via the DRAGEN Bio-IT platform, integrated with BaseSpace Sequence Hub, offering high-performance pipelines and fast processing of genetic variants, including point mutations, copy number variations and indels. For those who prefer on-site analysis, the Local Run Manager allows direct processing of sequences on the instrument.
The device features Illumina's SBS chemistry, based on reversible terminators, ensuring accurate nucleotide reads and limiting errors in complex sequences. The new v2.5 flow cells enhance fluorescence intensity stability, with improved tolerance to humidity and heat, ensuring consistent performance throughout the run.
In addition to sequencing, the NextSeq 550 offers BeadArray-type chip reading, compatible with Infinium technology. This makes it possible, for example,to identify methylated regions with the BeadChip EPIC or to quickly confirm CNVs with CytoSNP chips. Results can be analysed using GenomeStudio or open source tools under R, which are suitable for methylation research.
