Illumina NextSeq 550Dx – Clinical sequencing and research on a single platform
Illumina's NextSeq 550Dx is a medium-throughput sequencing system designed to provide clinical and research laboratories with a comprehensive, internationally compliant solution for genomic analysis. CE-IVD certified and FDA approved, it meets the strict requirements of diagnostic testing while offering a dedicated research mode, enabling the exploration of a wide range of scientific applications. Thanks to its dual functionality, laboratories can easily switch between standard diagnostic analyses and research projects, maximising the use of the instrument.
This platform delivers up to 90 gigabases of data in less than 35 hours, while maintaining a high level of accuracy. It is based on Illumina's synthesis-based sequencing (SBS) chemistry, recognised for its ability to detect individual bases with remarkable accuracy. This process reduces incorporation bias, improves coverage of repetitive and homogeneous regions, and enables reliable identification of rare or complex variants.
The NextSeq 550Dx is characterised by a three-step workflow: library preparation, sequencing and data analysis. Library preparation, performed with the Illumina DNA Prep with Enrichment Dx kit, requires only 50 ng of genomic DNA or DNA extracted from FFPE tissues and can be completed in less than a day. Pre-filled reagent cartridges make it easy to get started, reducing handling time to approximately 30 minutes per cycle.
In diagnostic mode, the instrument is optimised to work with validated IVD tests, ensuring reproducible results that comply with regulatory standards. Variant analysis can be automated via the dedicated DRAGEN server, which offers accurate detection of germline and somatic variants with a low error rate. In research mode, the NextSeq 550Dx supports a wide range of applications, from exome sequencing to transcriptomic analysis and customised targeted sequencing. It also enables DNA chip scanning (BeadChips) for genotyping, copy number variation assessment and methylation analysis.
The intuitive user interface and integrated software modules simplify sequence setup and analysis tracking. Users can schedule multiple sequencing runs, track progress in real time, and access results from the instrument or remotely. The solution also ensures data security and confidentiality, meeting the most stringent information protection standards.
The NextSeq 550Dx offers throughput flexibility with its Mid-Output and High-Output flow cell configurations. This allows sequencing capacity to be tailored to specific needs, whether for small sample batches or larger projects. Combined with the robustness of SBS technology and the reliability of Illumina software solutions, it is a strategic choice for laboratories seeking to combine performance, speed and versatility.
The NextSeq 550Dx is a unique solution that meets the demands of clinical diagnostics while opening up new possibilities for genomic research. Its ability to quickly integrate new applications, its ease of use and its high level of accuracy make it an essential tool for exploring and exploiting high-quality genetic data.
