NovaSeq 6000 Sequencing System - Good Condition 2017

NovaSeq 6000: Scalable sequencing for all genomic applications

Illumina's NovaSeq 6000 represents a major advance in high-throughput sequencing. Designed to meet the demands of the most ambitious research projects, it combines proven sequencing-by-synthesis (SBS) technology with a flexible architecture that allows data output, analysis time and cost per sample to be tailored to the specific needs of each study.

With four flow cell types (SP, S1, S2, S4), the NovaSeq 6000 allows you to modulate sequencing throughput, read length and the number of samples per run. This makes it possible to perform both low-intensity studies and large-scale whole genome sequencing projects. The system can operate with one or two flow cells simultaneously, delivering up to 6 terabytes of data and 20 billion reads in less than two days.

The NovaSeq 6000 offers two loading workflows: the standard workflow, with automated cluster generation on board, and the NovaSeq Xp workflow, which allows individual lane loading to separate projects or maximise multiplexing. The latter allows up to 384 samples to be loaded onto an S4 cell, while reducing the amount of DNA required.

Compatible with a wide range of Illumina library preparation kits, the NovaSeq 6000 supports a variety of methods such as whole genome sequencing, targeted sequencing, RNA-seq and epigenomics. It integrates seamlessly into a complete workflow including sample management via BaseSpace Clarity LIMS, automated library preparation, sequencing, data analysis with BaseSpace Sequence Hub and DRAGEN Bio-IT Platform, and interpretation via TruSight Software Suite.

Its ingenious design is based on nanoparticle flow cell technology, high-resolution optics and exclusion amplification, ensuring high cluster density and optimal data quality. RFID consumables, ready-to-use cartridges and an intuitive touchscreen interface simplify use and reduce handling time.

Cutting-edge technology for exceptional data quality

The NovaSeq 6000 uses Illumina's SBS technology with reversible terminators, enabling parallel sequencing of billions of DNA fragments. This method reduces homopolymer errors and ensures accurate base calling. The nanoparticle flow cells contain billions of fixed-position wells, optimising cluster spacing and increasing sequencing density.

The system's optics enable fast, high-resolution scanning, helping to make the NovaSeq 6000 the highest-throughput Illumina platform. Exclusion amplification maximises well occupancy by clusters from a single strand of DNA, significantly increasing data output.

Integrated, automated workflow for maximum efficiency

The NovaSeq 6000 integrates into a comprehensive sequencing ecosystem, including:

• BaseSpace Clarity LIMS: sample and reagent management, workflow automation, intuitive interface.
• Automated library preparation: Illumina-qualified methods with robotics partners.
• Data analysis: BaseSpace Sequence Hub, DRAGEN Bio-IT Platform, FASTQ file generation.
• Interpretation: TruSight Software Suite for genetic diseases, BaseSpace Correlation Engine for comparison with public databases.

SP Flow Cell

• Lines: 2
• Output:
  • 2 × 50 bp: 65–80 Gb
  • 2 × 100 bp: 134–167 Gb
  • 2 × 150 bp: 200–250 Gb
  • 2 × 250 bp: 325–400 Gb
• PE reads: 1.3–1.6 billion
• Quality: Q30 ≥85% (100/150 bp), Q30 ≥75% (250 bp)
• Run time: 13–38 hours depending on read length

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S1 flow cell

• Lines: 2
• Output:
  • 2 × 50 bp: 134–167 Gb
  • 2 × 100 bp: 266–333 Gb
  • 2 × 150 bp: 400–500 Gb
• PE reads: 2.6–3.2 billion
• Quality: Q30 ≥85%
• Run time: 13–25 hours

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S2 flow cell

• Lines: 2
• Output:
  • 2 × 50 bp: 333–417 Gb
  • 2 × 100 bp: 667–833 Gb
  • 2 × 150 bp: 1000–1250 Gb
• PE reads: 6.6–8.2 billion
• Quality: Q30 ≥85%
• Run time: 16–36 hours

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S4 flow cell

• Lines: 4
• Output:
  • 2 × 100 bp: 1600–2000 Gb
  • 2 × 150 bp: 2400–3000 Gb
• PE reads: 16–20 billion
• Quality: Q30 ≥85%
• Run time: 36–44 hours