7300: a versatile real-time PCR system for accurate and reproducible results
The 7300 system from Thermo Fisher Scientific, based on the expertise of Applied Biosystems, is a reliable platform for nucleic acid quantification and detection. By combining thermal cycling, optical detection and processing software, this system facilitates the analysis of the fluorescent signal produced at each PCR amplification cycle, providing real-time reading of the process without additional post-PCR steps.
Designed to meet the demands of research and diagnostic laboratories, the 7300 System supports volumes ranging from 20 to 100 µL in 96-well plates or individual 0.2 mL tubes. It is suitable for absolute or relative quantitative analyses as well as qualitative applications such as SNP genotyping or more/less testing with internal controls. Its ability to detect as few as 10 copies of target DNA with a high level of confidence underscores its sensitivity and accuracy.
The optics are based on a tungsten-halogen lamp with a CCD camera and filters optimised for fluorophores such as FAM™, VIC®, TAMRA™, NED™, ROX™, SYBR® Green I and JOE™, enabling four-colour multiparametric detection. Data collection is performed simultaneously on all filters for each well, with the option to modify the configuration after execution.
The Sequence Detection software, running on Windows XP, offers intuitive operation thanks to configuration wizards, real-time monitoring of amplification curves, and automated analysis tools. It also allows analysis on multiple plates, with direct export of results and customisable graphs.
This system guarantees high thermal uniformity and remarkable stability thanks to Peltier technology, with temperature accuracy of ±0.25°C. The platform includes lamp life monitoring and integrated diagnostics to ensure optimal operation.
The 7300 is therefore a comprehensive, robust tool that is easy to integrate into any real-time PCR workflow. It meets the needs of users who want a reliable and cost-effective solution for a wide range of molecular biology applications, while ensuring optimal traceability and reproducibility of results.
