The Applied Biosystems 7500 PCR Analyzer is an extremely versatile platform for the detection and quantification of nucleic acid sequences. By utilizing real-time PCR technology, it provides immediate quantitative results with exceptional sensitivity and accuracy, thus offering time savings and a wide dynamic range. An essential tool for both research and diagnostic applications.
The Applied Biosystems 7500 Real-Time PCR System stands at the forefront of molecular biology technology, offering enhanced performance capabilities and a pathway to high-speed thermal cycling. With its five-color detection system, this versatile platform allows for a wide range of applications, including gene expression analysis, SNP genotyping, and plus/minus assays utilizing internal positive controls.
Featuring an advanced optical configuration, the 7500 System supports a broader range of fluorophores, enabling the detection of various dyes with exceptional sensitivity and precision. Its variable excitation capability enhances sensitivity for longer wavelength (red) dyes, ensuring accurate and reliable results across different experimental setups.
The system's powerful and flexible software streamlines experimental setup with plate setup wizards, guiding users through the process effortlessly. Automated analysis tools, such as the RQ Study software, facilitate simultaneous analysis and visualization of data from multiple plates, enhancing efficiency and data interpretation.
In the field of real-time PCR, the Applied Biosystems 7500 System represents a significant advancement, offering immediate quantitative results without the need for post-PCR manipulation. Compared to traditional quantitation techniques, such as Northern blotting or RNase protection assays, real-time PCR delivers substantial time savings, superior sensitivity, precision, and a wider dynamic range.
Equipped with fast thermal cycling capabilities, the 7500 Fast System configuration enables high-speed PCR runs in approximately 35 minutes, making it ideal for time-sensitive experiments. Additionally, the system supports various thermal cycling modes, including Fast, Standard, and 9600 emulation, ensuring compatibility with diverse experimental requirements.
With its fluorescence detection system, the 7500 System illuminates sample wells with a tungsten halogen lamp and employs excitation and emission filters optimized for different fluorophores. This setup enhances sensitivity and precision, even in fast thermal cycling mode, ensuring accurate detection and quantification of nucleic acid sequences.
In summary, the Applied Biosystems 7500 Real-Time PCR System offers researchers and diagnosticians a versatile and efficient tool for a wide range of molecular biology applications. Its advanced features, rapid thermal cycling, and precise detection capabilities make it an indispensable asset in laboratories worldwide.
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