Real-Time PCR with the CFX96 System
The Bio-Rad CFX96 Real-Time PCR Detection System represents a significant advancement in qPCR technology, designed to enhance the precision and efficiency of your real-time PCR experiments. This system integrates seamlessly with the C1000 Touch Thermal Cycler, converting it into a state-of-the-art real-time PCR instrument. It offers a six-channel optical system—comprising five color channels and one FRET (Fluorescence Resonance Energy Transfer) channel—that provides exceptional versatility and sensitivity for a wide range of applications.
The CFX96 system is engineered with cutting-edge optical technology and precise thermal control to ensure accurate and reliable results. The six-channel configuration allows for the detection of up to five different targets simultaneously, making it ideal for complex multiplex assays. The system’s advanced features include a rapid maximum ramp rate of 5°C/sec, ensuring that thermal cycling is performed with speed and efficiency. With an average ramp rate of 3.3°C/sec and precise temperature control, the system maintains exceptional uniformity and accuracy, crucial for reproducible and reliable results.
A standout feature of the CFX96 System is its integrated LCD touch screen, which simplifies experiment setup and real-time monitoring. Users can easily view amplification traces and make adjustments on the fly, enhancing both convenience and efficiency. For more detailed analysis and data management, the included CFX Maestro Software provides a user-friendly interface for designing experiments, analyzing results, and exporting data. This software supports up to five-target multiplexing and offers advanced data analysis tools, including normalized gene expression calculations.
The system also features a thermal gradient capability, allowing for the optimization of reaction conditions in a single run. This feature is particularly useful for identifying the optimal annealing temperature for PCR reactions. Furthermore, the CFX96 System minimizes sample and reagent use with its low-volume requirement of just 10 µl and supports high-throughput capabilities through integration with the CFX Automation System II. This flexibility enables users to scale their operations according to their specific needs.
For regulatory compliance, the CFX96 System can be used in conjunction with CFX Maestro Software, Security Edition, which simplifies adherence to U.S. FDA 21 CFR Part 11 regulations. This ensures that your data collection and analysis processes meet the highest standards of data integrity and security.
The CFX96 Real-Time PCR Detection System also offers a range of configuration options. It can be operated stand-alone, without the need for a connected computer, or integrated with up to four systems from a single computer for expanded capacity. The system’s versatility extends to its accessories, including the CFX Qualification Plate and PX1 PCR Plate Sealer, which support various experimental needs and enhance overall performance.
In summary, the Bio-Rad CFX96 Real-Time PCR Detection System delivers unmatched performance, flexibility, and ease of use, making it an excellent choice for researchers seeking a powerful and reliable qPCR solution.
