PCR thermocycler MASTERCYCLER X50H - Good

Mastercycler X50H : Precision and Flexibility for PCR Applications

The Mastercycler X50H by Eppendorf represents a significant advancement in PCR technology, combining exceptional performance with unparalleled versatility. This high-capacity thermocycler is meticulously engineered to meet the demands of both sophisticated molecular biology research and routine PCR procedures.

At the heart of the Mastercycler X50H’s design is its ability to handle a wide range of sample types and volumes. It features a thermoblock that accommodates a 384-well PCR plate, making it an ideal choice for high-throughput applications. The device’s temperature control range spans from 4°C to 99°C, with a remarkable ramp rate of up to 5°C/s for heating and 2.3°C/s for cooling. This ensures rapid and efficient thermal cycling, crucial for optimizing complex PCR protocols.

One of the standout features of the Mastercycler X50H is its innovative 2D gradient capability. This function allows precise temperature gradient control along both the X and Y axes, facilitating the optimization of PCR conditions with high accuracy. Researchers can fine-tune annealing and denaturation temperatures to enhance the specificity and yield of their PCR reactions, making this feature particularly valuable for advanced molecular biology applications.

The Mastercycler X50H is equipped with a user-friendly touchscreen interface that simplifies operation and program management. The intuitive touch screen provides easy access to all settings and parameters, allowing for quick adjustments and streamlined protocol execution. 

The innovative flexlid® concept of the Mastercycler X50H ensures compatibility with a wide range of PCR consumables. The automatic height adjustment of the lid accommodates various types of consumables, from low-volume PCR tubes to 384-well plates, without the need for manual lid adjustments. This feature not only improves ease of use but also reduces the potential for contamination and ensures consistent sealing across different sample types.

The Mastercycler X50H supports multiple connectivity options, including USB and Ethernet interfaces. This allows for seamless integration with the VisioNize Lab Suite for enhanced laboratory management. Additionally, up to 50 units can be connected using the CycleManager X50 software, enabling centralized control and monitoring of multiple thermocyclers from a single workstation.

Despite its high capacity and advanced features, the Mastercycler X50H boasts a compact footprint, making it suitable for laboratories with limited space. Its energy-efficient design, with a maximum power consumption of 850 W, ensures lower operational costs and reduced environmental impact.

The Mastercycler X50H meets stringent safety and electromagnetic compatibility standards, including IEC 61326-1 Class B. It is designed for indoor use with environmental conditions specified to ensure reliable performance. The device features overvoltage protection, pollution degree 2, and a protection class I, ensuring robust safety for both the equipment and users.

The Mastercycler X50H by Eppendorf is a high-performance PCR thermocycler that excels in precision, flexibility, and ease of use. Its advanced features, including high-capacity thermoblocks, innovative temperature gradient function, and intuitive touchscreen interface, make it an indispensable tool for both cutting-edge research and routine PCR applications.

• Dimensions:
  • Width: 275 mm
  • Depth: 430 mm
  • Height (lid closed): 330 mm
  • Height (lid open): 439 mm
• Weight:
  • Mastercycler X50H: 11.5 kg
• Mains/Power Supply:
  • Voltage: 100 V - 240 V ± 10%
  • Frequency: 50 Hz - 60 Hz
  • Power Consumption: 850 W max.
  • Overvoltage Category: II
  • Pollution Degree: 2
  • Protection Class: I
• Interfaces:
  • USB 1x 2.0 Type A
  • Ethernet:
    • 1x RJ-45 for VisioNize Lab Suite
    • 1x RJ-45 for Cycler network
    • Compatibility: IEEE 802.3, 10/100 MBit/s or 10/100/1000 MBit/s
• Thermoblock:
  • Capacity: 1x 384-well PCR plate
  • Temperature Control Range: 4°C – 99°C
  • Heating Ramp Rate: Max. 5°C/s
  • Cooling Ramp Rate: Max. 2.3°C/s
  • Temperature Homogeneity:
    • ±0.2°C (20°C – 72°C)
    • ±0.3°C (72°C – 95°C)
  • Control Accuracy: ±0.15°C
  • Gradient Spread: 30°C max.
  • Gradient Temperature Control Range: 30°C – 99°C
  • Heated Lid Temperature Control Range: 37°C – 110°C
• Noise Levels:
  • Idle: < 31.2 dB(A)
  • Default PCR Execution: < 33.6 dB(A)
  • Continuous Cooling Cycle at 4°C: < 47.2 dB(A)