PCRmax Eco 48 Thermal Cycler - Good Condition

High-performance real-time PCR system for research

The PCRmax Eco 48 is a real-time PCR (qPCR) instrument designed for molecular biology research laboratories. It enables real-time amplification, detection and quantification of DNA using a technology that combines an innovative thermal system with precise optical detection. This system is intended for research use only and offers a complete solution for quantification, genotyping and high-resolution melting curve (HRM) analysis.

Its hollow silver thermal block, filled with conductive fluid, ensures exceptional thermal uniformity across all 48 wells. This technology allows 40 PCR cycles to be performed in less than 40 minutes, while guaranteeing an accuracy of ±0.1°C. The optical system is based on two sets of LEDs and a CCD camera equipped with four filters, enabling the detection of fluorophores such as SYBR® Green, FAM™, HEX™, VIC™, ROX™, Cy®5 and Q670™. This configuration ensures reliable and reproducible data acquisition in every PCR cycle.

Versatile applications: quantification, genotyping and HRM

The PCRmax Eco 48 supports several types of experiments, tailored to the diverse needs of researchers. Absolute quantification is based on a standard curve obtained by serial dilutions of a sample of known concentration. It allows the Cq values of unknown samples to be interpolated with high accuracy. Relative quantification measures the expression of a target gene relative to a reference sample, with normalisation by a stable reference gene.

The system also allows genotyping by allele discrimination, using hydrolytic probes labelled with distinct fluorophores (usually FAM and VIC). This method identifies homozygotes for each allele as well as heterozygotes, based on the signals detected. HRM analysis offers a cost-effective and efficient alternative for detecting genetic variations, such as SNPs and mutations, without the need for specific probes. It is based on the progressive fusion of amplicons after amplification, and the interpretation of the fusion curves allows genetic profiles to be distinguished.

Multiplexing is another key feature of PCRmax Eco 48. It allows the simultaneous detection of multiple targets in a single reaction, thanks to its four optical channels. This capability optimises sample utilisation, reduces costs and improves the accuracy of results, in particular through the integration of internal controls for normalisation.

Optimised workflow and intuitive software interface

The workflow with the PCRmax Eco 48 is designed to be smooth and efficient. After preparing the sample plate and launching the software, the user can define their experiment by selecting the application type, detection chemistry, starting material and quantification method. The software offers default thermal profiles, which can be modified as needed, and allows the well layout to be configured by assigning assays, samples and standards.

During PCR, the software displays amplification curves and data broken down by well in real time. Once the analysis is complete, the results can be exported in the desired format. The system also includes data recovery functions in case of connection loss, as well as diagnostic and maintenance tools.

• Sample format: 48-well plate
• Reaction volume: 5–20 µl
• Preheating time: ~20 minutes
• Typical PCR time: < 40 minutes for 40 cycles
• Sensitivity: single-copy detection
• Thermal uniformity: ± 0.1°C
• HRM resolution: up to 0.1°C
• Multiplexing: up to 4 simultaneous targets
• Light source: 2 sets of LEDs (452–486 nm and 542–582 nm)
• Detector: CCD camera with 4 filters (505–545 nm, 562–596 nm, 604–644 nm, 665–705 nm)
• Dimensions: 34.5 cm (W) x 31 cm (D) x 32 cm (H)
• Weight: 13.6 kg (with power supply)
• Power supply: 100–240 VAC, 50–60 Hz, 5A
• Operating temperature: 15°C to 40°C
• Operating humidity: 15–90% RH
• Protection: IP20