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QX200 Droplet Digital PCR System - Good Condition

The Bio-Rad QX200 Droplet Digital PCR System is an innovative solution for absolute quantification of DNA and RNA. Using microfluidic technology and nanolitre droplet generation, it delivers accurate and reproducible results without the need for a standard curve. Suitable for a wide range of applications such as mutation detection, biomarker analysis and gene expression studies, this system offers high throughput and high sensitivity to meet the demands of modern research laboratories.



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BioRad QX200 Droplet

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Description

Bio-Rad's QX200 Droplet Digital PCR System: precision for molecular research

Bio-Rad's QX200 Droplet Digital PCR System is designed to revolutionise the way scientists quantify DNA and RNA molecules. This technology is based on an innovative concept: the partitioning of the sample into approximately 20,000 nanolitre droplets that serve as independent micro-reactors. Each droplet is analysed for the presence of the target molecule, enabling absolute quantification according to a Poisson distribution. This method eliminates the need for calibration curves and offers unrivalled accuracy, even for targets present in very low abundance.

The QX200 system consists of two main instruments: a droplet generator and a droplet reader. The generator partitions the sample into a water-oil emulsion to create uniform droplets. After PCR amplification, these droplets are analysed individually by the reader, which measures their fluorescence. Positive droplets indicate the presence of the target sequence, while negative droplets do not. The analysis is performed using QuantaSoft software, which provides a statistical interpretation of the collected data.

This solution stands out for its flexibility and ability to adapt to workflows of varying complexity. It can process eight samples simultaneously and scale up to 96 samples in just five hours, with minimal handling time. Its modular architecture allows throughput to be increased by multiplying the number of experiments from 96 samples in a single day, which is ideal for environments with a high work rate.

The QX200 Droplet Digital PCR System excels in a variety of applications. In cancer research, it can detect rare mutations and quantify biomarkers at extremely low concentrations, down to a ratio of 1 in 1,000,000 total copies. This remarkable sensitivity makes it essential for viral reservoir analysis, validation of next-generation sequencing libraries , and the study of genomic copy number variations. Its compatibility with hydrolysis probes and EvaGreen chemistry further expands its capabilities, from simple detection to complex gene expression analyses.

The system meets the needs of researchers who want robust, reproducible and easily interpretable data. Its intuitive user interface and ergonomic design simplify experiment preparation, reducing the risk of errors and speeding up analysis. The QX200 is therefore an indispensable asset for molecular biology, genomics and oncology laboratories seeking to push the boundaries of nucleic acid detection and analysis.

Features

  • Absolute quantification of nucleic acids without a standard curve
  • Generation of homogeneous nanolitre droplets for precise sample partitioning
  • Statistical analysis via QuantaSoft software for reliable results
  • Compatibility with hydrolysis probes and EvaGreen chemistry for a wide range of applications
  • Modular throughput allowing analysis of 96 samples in 5 hours
  • High sensitivity enabling detection of rare mutations and low-abundance nucleic acids
  • Ergonomic design reducing handling time and simplifying workflows
  • Can be used for NGS library quantification and post-sequencing validation


Technical Details

  • Starting sample volume: 20 µl
  • Droplet generator capacity: 1 to 8 samples per cartridge
  • Number of droplets generated per sample: approximately 20,000
  • Droplet generator dimensions: 28 x 36 x 13 cm
  • Droplet reader accuracy: ±10%
  • Linear dynamic range: 5 orders of magnitude
  • Droplet reader capacity: 1 to 96 samples per plate
  • Number of droplets per 96-well plate: approx. 1,500,000
  • Sample illumination system: light-emitting diodes (LEDs)
  • Detection: multipixel photon counter
  • Detection channels: FAM (EvaGreen), HEX (VIC)
  • Droplet reader dimensions: 66 x 52 x 29 cm

Compatible Accessories

  • DG8 cartridges for droplet generator
  • DG8 seals for droplet generator
  • DG8 cartridge holders
  • Droplet generation oils for probes and EvaGreen
  • Oil for droplet reader (1 L cans)
  • Supermix ddPCR for probes (various formats available)
  • One-Step RT-ddPCR kits for probes
  • EvaGreen ddPCR Supermix (various formats available)
  • ddPCR library quantification kits for Illumina TruSeq and Ion Torrent
  • C1000 Touch thermal cycler with 96-well reaction module
  • PX1 PCR plate sealer

Logistics

Remma and its partners provide you with serviced and configured equipment tailored to your needs. Receive your product at your facility with standard or express delivery, and an installation option, all through a single point of contact.

Warranty

Our 12/24-month warranty ensures the safety and reliability of your medical equipment throughout its lifespan.

Flexible Financing

Settle your medical equipment in up to 36 installments with our financing service or pay after delivery with Remma Facility.