CFX Opus 96: Efficiency and Simplicity for Your Real-Time PCR Analyses
The CFX Opus 96 Real-Time PCR System from Bio-Rad provides laboratories with a robust and precise instrument designed to meet the demands of real-time PCR applications. Its user-friendly touchscreen interface and compatibility with CFX Maestro™ software make experiment setup and data analysis seamless. The software offers powerful tools for quantitative analysis, melt curve analysis, relative gene expression, and endpoint analysis. The system supports the detection of up to five different targets per well, providing true flexibility in experimental design.
With integrated connectivity options (Wi-Fi, Ethernet, and USB), the CFX Opus 96 can operate as a standalone unit or be fully integrated into a laboratory network infrastructure. The BR.io cloud platform enables secure remote access to data, allowing users to start, monitor, and analyze experiments from virtually anywhere. This flexibility is enhanced by cross-platform software compatibility, with full functionality on Windows and analysis capability on macOS.
The thermal performance of the CFX Opus 96 is built on a 96-well fixed-block design with a Peltier-based heating/cooling system, ensuring high thermal uniformity and fast ramp rates. The system offers temperature accuracy of ±0.2 °C and maximum uniformity of ±0.3 °C, which are critical for ensuring result reproducibility.
For optical detection, the system utilizes six filtered LEDs for excitation and six filtered photodiodes for detection, covering a broad wavelength range from 450 to 730 nm. A full-plate scan is completed in 12 seconds, with single-channel reads in as little as 3 seconds. The system is capable of detecting as few as one copy of target sequence in human genomic DNA, ensuring high sensitivity for the most demanding applications.
Designed for both novice and experienced users, the CFX Opus 96 simplifies the creation and editing of custom protocols. Experiments can be saved directly to network folders, results can be automatically emailed, and user access can be secured via login credentials. This high level of customization supports efficient organization, complete traceability, and compliance with the standards of research and diagnostic laboratories.
