Real-Time PCR System CFX Opus 96 Dx - Fair Condition

CFX Opus 96 Dx: A Reliable and High-Performance PCR Platform for In Vitro Diagnostic Applications

The CFX Opus 96 Dx from Bio-Rad is a real-time PCR system specifically designed to ensure high-quality and reliable results in clinical and regulated environments. Compliant with in vitro diagnostic (IVD) requirements, this thermal cycler delivers comprehensive data management, outstanding thermal uniformity, and excellent sensitivity—meeting laboratories’ expectations for traceability, accuracy, and reproducibility.

Equipped with an intuitive, adjustable touchscreen interface, the CFX Opus 96 Dx simplifies protocol setup, whether creating new methods or adapting existing programs. It also offers advanced user management with customizable security settings, including individual login credentials and password protection. The accompanying CFX Maestro Dx SE software provides robust regulatory support, including features such as audit trails, data logging, and electronic signatures in compliance with 21 CFR Part 11.

The system stands out for its consistent and stable thermal performance. It ensures temperature uniformity of ±0.3 °C well-to-well, a critical factor for real-time PCR amplification. Its multiplexing capability enables the detection of up to five distinct targets per well, while maintaining exceptional sensitivity—capable of detecting a single copy of a target sequence in human genomic DNA. This makes it particularly suitable for laboratories developing or performing complex assays with demanding performance requirements.

Thanks to compatibility with Windows 10 (64-bit) and multiple connectivity options (USB and Ethernet), the CFX Opus 96 Dx integrates seamlessly into laboratory information systems. Up to four instruments can be operated simultaneously from a single software interface, offering improved efficiency and workflow organization for amplification cycles.

Data export is both simple and comprehensive, with customizable report generation, multiple output formats, and high-resolution image capture. The CFX Opus 96 Dx is a powerful, secure, and user-friendly solution for laboratories implementing real-time PCR assays within the framework of in vitro diagnostic testing.

• Capacity: 96 wells
• Sample Volume: 1 to 50 µL (10–50 µL recommended)
• Interface: Adjustable touchscreen (12–55° tilt range)
• Dimensions: 33 × 56 × 36 cm
• Weight: 22 kg
• Connectivity: USB 2.0, Ethernet
• Operating System: Windows 10 IoT
• Optical Detection: 6 filtered LEDs / 6 filtered photodiodes
• Wavelength Range: 450–730 nm
• Sensitivity: Detection of a single copy of human genomic DNA
• Multiplexing: Up to 5 targets per well
• Scan Speed: 3 seconds (single channel) / 12 seconds (all channels)
• Thermal Gradient Range: 1 to 24 °C across 30–100 °C
• Thermal Uniformity: ±0.3 °C
• Thermal Accuracy: ±0.2 °C
• Heating System: Peltier-based with heated lid (30–110 °C)
• Temperature Range: 4–100 °C