StepOnePlus™: Precision Performance for Your Real-Time Analyses
The StepOnePlus™ System from Thermo Fisher Scientific is designed to provide an accessible yet high-performing solution for laboratories seeking a reliable instrument capable of conducting 96-well quantitative PCR (qPCR) analyses. It caters to both novice users and experienced researchers, thanks to its user-friendly software interface that guides users through each step of the experiment—from plate setup to result interpretation.
A key feature of the StepOnePlus™ is its VeriFlex™ technology, which is based on six independently controlled Peltier blocks. This unique design enables differential thermal control, tailored to the specific requirements of primers and probes, thereby enhancing performance in multiplex PCR assays. The system also supports fast cycling protocols, delivering results in under 40 minutes to maximize daily throughput.
Fluorescence detection is driven by a long-lasting LED-based optical system, capable of reading four fluorophores (FAM/SYBR Green I, VIC/JOE, ROX, and TAMRA), significantly broadening the range of supported applications. Reactions can be monitored in real time via the built-in touchscreen display or remotely through a network-connected PC, with email notifications sent upon completion of the runs.
The integrated software supports a wide range of analysis modes, including absolute and relative quantification, SNP genotyping, presence/absence detection, and melt curve analysis. It also features automated quality control, which flags potential data anomalies to ensure reliable and rigorous interpretation.
The StepOnePlus™ can operate in standalone mode using its LCD screen and USB ports, or be connected to a PC or network, offering complete flexibility for various laboratory workflows. Compact, precise, and easy to use, it is a valuable asset for real-time PCR applications that demand accuracy, speed, and adaptability.
