Reference 2 Single-Channel Variable Volume Pipette

Reference® 2 Single-Channel Variable Volume Pipettes – Flexible Precision for the Laboratory

The Eppendorf Reference® 2 single-channel variable volume pipette has been designed to meet the precision, flexibility and reliability requirements of the most demanding laboratory environments. Operating on an air cushion principle, it allows dosing without contact between the liquid and the internal components, ensuring better hygiene and simplified maintenance. Its adjustable volume range, depending on the model, offers great versatility in everyday liquid handling tasks. It is suitable for both routine tasks and more specific protocols requiring a wide range of volumes.

Equipped with an intuitive and secure volume locking system, it prevents handling errors and guarantees reproducible results. Its multifunction push button allows the volume to be adjusted, the liquid to be aspirated, dispensed and the tip ejected, while reducing user effort. This ergonomic design allows for a natural grip, suitable for long pipetting sessions, without causing strain on the wrist or fingers.

The components of the Reference 2 are made of robust, chemical-resistant materials: refined polypropylene, PVDF, PEI, stainless steel , etc. This choice not only ensures the device's long service life, but also protects it from aggressive agents commonly used in laboratories. Each pipette is also equipped with an integrated RFID chip, enabling traceability and integration into equipment management systems.

The Reference® 2 is fully autoclavable (at 121°C for 20 minutes) without the need for prior disassembly. It is also UV sterilisable, making it ideal for environments requiring a high level of hygiene, such as microbiology, molecular biology and quality control laboratories.

Its design is also optimised for thorough cleaning. The lower body can be dismantled, allowing direct access to critical components for maintenance or decontamination operations. Compatible with epT.I.P.S. tips, it ensures a perfect fit to limit volume loss and enhance dosing accuracy.

Whether for one-off applications or long series, the Reference® 2 pipette is a reliable, robust and comfortable tool that helps improve experimental rigour and laboratory efficiency.

0.1 – 2.5 µL:

• Volume range: 0.1 – 2.5 μL
• Increment: 0.002 μL
• Colour code: dark grey
• Recommended tip: epT.I.P.S. 34 mm
• Control volume (0.1 μL): Systematic error: ±48.0% (±0.048 μL); Random error: ±12.0% (±0.012 μL)
• Control volume (0.25 μL): Systematic error: ±12.0% (±0.03 μL); Random error: ±6.0% (±0.015 μL)
• Control volume (1.25 μL): Systematic error: ±2.5% (±0.031 μL); Random error: ±1.5% (±0.019 μL)
• Control volume (2.5 μL): Systematic error: ±1.4% (±0.035 μL); Random error: ±0.7% (±0.018 μL)

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0.5 – 10 µL:

• Volume range: 0.5 – 10 μL
• Increment: 0.01 μL
• Colour code: medium grey
• Recommended tip: epT.I.P.S. 40 mm
• Control volume (0.5 μL): Systematic error: ±8.0% (±0.04 μL); Random error: ±5.0% (±0.025 μL)
• Control volume (1 μL): Systematic error: ±2.5% (±0.025 μL); Random error: ±1.8% (±0.018 μL)
• Control volume (5 μL): Systematic error: ±1.5% (±0.075 μL); Random error: ±0.8% (±0.04 μL)
• Control volume (10 μL): Systematic error: ±1.0% (±0.10 μL); Random error: ±0.4% (±0.04 μL)

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2–20 µL:

• Volume range: 2 – 20 μL
• Increment: 0.02 μL
• Colour code: light grey
• Recommended tip: epT.I.P.S. 46 mm or 53 mm
• Control volume (2 μL): Systematic error: ±3.0% (±0.06 μL); Random error: ±1.5% (±0.03 μL)
• Control volume (10 μL): Systematic error: ±1.0% (±0.10 μL); Random error: ±0.6% (±0.06 μL)
• Control volume (20 μL): Systematic error: ±0.8% (±0.16 μL); Random error: ±0.3% (±0.06 μL)

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2–20 µL:

• Volume range: 2 – 20 μL
• Increment: 0.02 μL
• Colour code: yellow
• Recommended tip: epT.I.P.S. 46 mm or 53 mm
• Control volume (2 μL): Systematic error: ±3.0% (±0.06 μL); Random error: ±1.5% (±0.03 μL)
• Control volume (10 μL): Systematic error: ±1.0% (±0.10 μL); Random error: ±0.6% (±0.06 μL)
• Control volume (20 μL): Systematic error: ±0.8% (±0.16 μL); Random error: ±0.3% (±0.06 μL)

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10–100 µL:

• Volume range: 10 – 100 μL
• Increment: 0.1 μL
• Colour code: yellow
• Recommended tip: epT.I.P.S. 53 mm
• Control volume (10 μL): Systematic error: ±3.0% (±0.3 μL); Random error: ±0.7% (±0.07 μL)
• Control volume (50 μL): Systematic error: ±1.0% (±0.5 μL); Random error: ±0.3% (±0.15 μL)
• Control volume (100 μL): Systematic error: ±0.8% (±0.8 μL); Random error: ±0.2% (±0.2 μL)

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20 – 200 µL:

• Volume range: 20 – 200 μL
• Increment: 0.2 μL
• Colour code: yellow
• Recommended tip: epT.I.P.S. 53 mm
• Control volume (20 μL): Systematic error: ±2.5% (±0.5 μL); Random error: ±0.7% (±0.14 μL)
• Control volume (100 μL): Systematic error: ±1.0% (±1.0 μL); Random error: ±0.3% (±0.3 μL)
• Control volume (200 μL): Systematic error: ±0.6% (±1.2 μL); Random error: ±0.2% (±0.4 μL)

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30 – 300 µL:

• Volume range: 30–300 μL
• Increment: 0.2 μL
• Colour code: orange
• Recommended tip: epT.I.P.S. 55 mm
• Control volume (30 μL): Systematic error: ±1.5% (±0.45 μL); Random error: ±0.6% (±0.18 μL)
• Control volume (300 μL): Systematic error: ±0.6% (±1.8 μL); Random error: ±0.2% (±0.6 μL)
• Technology: air cushion piston
• Display: adjustable digital

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100 – 1000 µL:

• Volume range: 100 – 1000 μL
• Increment: 1 µL
• Colour code: blue
• Recommended tip: epT.I.P.S. 71 mm
• Control volume (100 μL): Systematic error: ±3.0% (±3.0 μL); Random error: ±0.6% (±0.6 μL)
• Control volume (1000 μL): Systematic error: ±0.6% (±6.0 μL); Random error: ±0.2% (±2.0 μL)
• Technology: air cushion piston
• Display: adjustable digital

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0.25 – 2.5 mL:

• Volume range: 0.25 – 2.5 mL
• Increment: 0.002 mL
• Colour code: red
• Recommended tip: epT.I.P.S. 115 mm
• Control volume (0.25 mL): Systematic error: ±4.8% (±12 μL); Random error: ±1.2% (±3 μL)
• Control volume (2.5 mL): Systematic error: ±0.6% (±15 μL); Random error: ±0.2% (±5 μL)
• Technology: air cushion piston
• Display: adjustable digital

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0.5 – 5 mL:

• Volume range: 0.5 – 5 mL
• Increment: 0.005 mL
• Colour code: purple
• Recommended tip: epT.I.P.S. 120 mm
• Control volume (0.5 mL): Systematic error: ±2.4% (±12 μL); Random error: ±0.6% (±3 μL)
• Control volume (5 mL): Systematic error: ±0.6% (±30 μL); Random error: ±0.15% (±7.5 μL)
• Technology: air cushion piston
• Display: adjustable digital

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1 – 10 mL:

• Volume range: 1–10 mL
• Increment: 0.01 mL
• Colour code: turquoise
• Recommended tip: epT.I.P.S. 165 mm
• Control volume (1 mL): Systematic error: ±3.0% (±30 μL); Random error: ±0.6% (±6 μL)
• Control volume (10 mL): Systematic error: ±0.6% (±60 μL); Random error: ±0.15% (±15 μL)
• Technology: air cushion piston
• Display: adjustable digital