Rotor-Gene Q real-time PCR thermocycler

Rotor-Gene Q: exceptional precision for your real-time PCR analyses

The Rotor-Gene Q from Qiagen is a real-time PCR cycler that meets the most stringent requirements for reproducibility, speed and reliability of results. Unlike traditional thermal block instruments, this model is based on centrifugal rotation technology that allows each tube to rotate in a moving air chamber. This design eliminates temperature gradients and ensures that each sample is subjected to the same thermal conditions in every cycle, significantly reducing variations between wells.

Optical uniformity is ensured by direct line detection. Each tube is illuminated individually and the fluorescent signal is captured through a very short optical path. This configuration provides increased sensitivity while eliminating the need for reference dyes such as ROX. The Rotor-Gene Q supports up to six excitation and detection channels, covering a range from UV to infrared wavelengths, making it compatible with a wide variety of probes and dyes.

The flexibility of this system is also reflected in the reagent formats it accepts. It can accommodate standard PCR tubes, tube strips, and high-throughput Rotor-Discs with up to 100 wells. It is fully compatible with the QIAgility robot for automated reaction preparation, reducing the risk of human error.

The Rotor-Gene Q is particularly suitable for HRM (High Resolution Melting) applications, thanks to its ability to detect temperature variations of 0.02°C and its dedicated HRM channel. It is also effective for genotyping, DNA methylation analysis, viral detection and microRNA research.

Its intuitive software integrates a comprehensive range of analysis methods, including absolute or relative quantification, endpoint analysis, allele discrimination and more. Each results file is digitally signed, and audit trails ensure complete traceability of changes.

This system is designed for minimal maintenance: it requires no optical alignment or regular replacement of light sources. Lightweight and compact, it fits easily into any type of laboratory. The Rotor-Gene Q is therefore a strategic choice for demanding researchers seeking efficiency and reliability on a daily basis.

• Dimensions: 370 mm (W) × 420 mm (D) × 275 mm (H); depth with door open: 560 mm
• Weight: 14 kg
• Temperature uniformity: ±0.01°C
• Temperature accuracy: ±0.25°C
• Thermal resolution: ±0.02°C
• Max. temperature: 99°C
• Equilibration time: 0 s
• Ramping (heating/cooling): >15°C/s (heating), >20°C/s (cooling)
• Optical system: 6 channels (365–680 nm excitation; 460–750 nm detection)
• Detection: photomultiplier with adjustable gain, dynamic range over 10 orders of magnitude
• Configurations: Rotor-Disc 100: 30 µl × 100 wells; Rotor-Disc 72: 0.1 ml × 72 wells; 0.1 ml tubes: 10–30 µl (in strips of 4); 0.2 ml tubes: 15–50 µl (individual)
• Typical cycle time: 45–60 minutes
• Power supply: 100–240 V AC; 50/60 Hz; 560 VA