JA-20 Fixed Angle Rotor – Maximum Performance for Laboratory Centrifugation
The Beckman Coulter JA-20 Fixed Angle Rotor is engineered for laboratories that require high-speed centrifugation to separate subcellular particles, bacteria, and other biological materials. Designed to operate at speeds up to 20,000 rpm, this rotor ensures efficient separation with unparalleled reliability and performance, making it an ideal choice for research and clinical laboratories.
The JA-20 rotor can accommodate up to eight 50 mL tubes positioned at a 34-degree angle relative to the axis of rotation. This design enhances the pelleting process by applying centrifugal forces at an optimal angle for sedimenting subcellular particles such as membranes, organelles, and bacteria. Additionally, the rotor supports the use of gradient separation techniques, allowing up to 400 mL of gradient and sample to be processed in each run. Short-column methods, such as partially filled tubes, can also be employed to isolate and separate different classes of subcellular components.
The rotor’s impressive speed capabilities are matched by its smooth operation. Capable of generating up to 48,400 x g at a maximum speed of 20,000 rpm, the JA-20 rotor ensures optimal force distribution across the sample, leading to highly effective separation processes. With three different relative centrifugal field values (at maximum, average, and minimum radii), it offers users flexibility in their experimental approaches. Additionally, the JA-20 has a critical speed range of 600 to 800 rpm, ensuring it avoids vibrations during operation.
The rotor’s k-factor of 769 further optimizes pelleting performance, particularly for differential centrifugation techniques. This allows the JA-20 to quickly sediment particles, minimizing the overall runtime of the centrifugation process.
The JA-20 rotor is crafted from anodized aluminum, which provides excellent resistance to corrosion and wear, ensuring long-term durability. The rotor is anodized black, while the lid is blue for easy identification. O-rings made from Buna N rubber, installed in both the rotor body and lid, maintain atmospheric pressure within the rotor during centrifugation, ensuring sample integrity and safety. When properly lubricated, these O-rings provide an airtight seal, making the rotor highly reliable for sensitive biological applications.
The JA-20 also includes advanced safety features. Two vertical pins in the rotor drive hole prevent slippage by securely mating with grooves in the centrifuge drive hub. Whether used with older or newer model centrifuges, this mechanism ensures the rotor remains securely in place during both acceleration and deceleration.
This rotor is fully compatible with Beckman Coulter J2, J6, and Avanti J-series centrifuges. The rotor’s entry code is 20.2 for J2 and J6 series microprocessor-controlled centrifuges, and 20 for Avanti J-E centrifuges, facilitating seamless integration with these systems. Its approximate acceleration time to maximum speed is 1 ½ minutes when fully loaded, and deceleration takes about 2 ½ minutes, ensuring a swift and efficient centrifugation process.
Whether for research, clinical diagnostics, or industrial use, the Beckman Coulter JA-20 rotor offers a robust, high-performance solution for centrifugation. With its combination of speed, safety, and durability, it is an indispensable tool for laboratories that demand accuracy and reliability.