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Rotor JA-20 - Good

Categories: Laboratory


 The Beckman Coulter JA-20 Fixed Angle Rotor, rated for speeds up to 20,000 rpm, is designed to accommodate up to eight 50 mL tubes at a 34-degree angle for effective centrifugation. This rotor is ideal for pelleting bacteria, cell membranes, and subcellular particles, as well as for gradient centrifugation. Compatible with J2, J6, and Avanti J series centrifuges, it delivers exceptional reliability and precision.

Description

JA-20 Fixed Angle Rotor – Maximum Performance for Laboratory Centrifugation

The Beckman Coulter JA-20 Fixed Angle Rotor is engineered for laboratories that require high-speed centrifugation to separate subcellular particles, bacteria, and other biological materials. Designed to operate at speeds up to 20,000 rpm, this rotor ensures efficient separation with unparalleled reliability and performance, making it an ideal choice for research and clinical laboratories.

The JA-20 rotor can accommodate up to eight 50 mL tubes positioned at a 34-degree angle relative to the axis of rotation. This design enhances the pelleting process by applying centrifugal forces at an optimal angle for sedimenting subcellular particles such as membranes, organelles, and bacteria. Additionally, the rotor supports the use of gradient separation techniques, allowing up to 400 mL of gradient and sample to be processed in each run. Short-column methods, such as partially filled tubes, can also be employed to isolate and separate different classes of subcellular components.

The rotor’s impressive speed capabilities are matched by its smooth operation. Capable of generating up to 48,400 x g at a maximum speed of 20,000 rpm, the JA-20 rotor ensures optimal force distribution across the sample, leading to highly effective separation processes. With three different relative centrifugal field values (at maximum, average, and minimum radii), it offers users flexibility in their experimental approaches. Additionally, the JA-20 has a critical speed range of 600 to 800 rpm, ensuring it avoids vibrations during operation.

The rotor’s k-factor of 769 further optimizes pelleting performance, particularly for differential centrifugation techniques. This allows the JA-20 to quickly sediment particles, minimizing the overall runtime of the centrifugation process.

The JA-20 rotor is crafted from anodized aluminum, which provides excellent resistance to corrosion and wear, ensuring long-term durability. The rotor is anodized black, while the lid is blue for easy identification. O-rings made from Buna N rubber, installed in both the rotor body and lid, maintain atmospheric pressure within the rotor during centrifugation, ensuring sample integrity and safety. When properly lubricated, these O-rings provide an airtight seal, making the rotor highly reliable for sensitive biological applications.

The JA-20 also includes advanced safety features. Two vertical pins in the rotor drive hole prevent slippage by securely mating with grooves in the centrifuge drive hub. Whether used with older or newer model centrifuges, this mechanism ensures the rotor remains securely in place during both acceleration and deceleration.

This rotor is fully compatible with Beckman Coulter J2, J6, and Avanti J-series centrifuges. The rotor’s entry code is 20.2 for J2 and J6 series microprocessor-controlled centrifuges, and 20 for Avanti J-E centrifuges, facilitating seamless integration with these systems. Its approximate acceleration time to maximum speed is 1 ½ minutes when fully loaded, and deceleration takes about 2 ½ minutes, ensuring a swift and efficient centrifugation process.

Whether for research, clinical diagnostics, or industrial use, the Beckman Coulter JA-20 rotor offers a robust, high-performance solution for centrifugation. With its combination of speed, safety, and durability, it is an indispensable tool for laboratories that demand accuracy and reliability.

Features

  • High-Speed Centrifugation: The JA-20 rotor achieves a maximum speed of 20,000 rpm, making it ideal for demanding centrifugation tasks involving bacteria, subcellular particles, and gradient separation.
  • Optimal Pelleting Efficiency: With a 34-degree angle, this fixed-angle rotor optimizes pelleting efficiency for various biological materials, including cells and organelles.
  • Centrifugal Force: Generates up to 48,400 × g, ensuring rapid and efficient sample processing.
  • Durable and Corrosion-Resistant: Constructed from anodized aluminum, the JA-20 rotor is built for long-term use in demanding environments, offering resistance to corrosion and wear.
  • Buna N O-Rings: Rubber O-rings in the rotor and lid ensure atmospheric pressure control during high-speed operation, preserving sample integrity.
  • Safety Mechanisms: Features vertical pins that mate securely with the centrifuge drive hub, preventing rotor slippage during acceleration and deceleration.
  • Compatibility: Works with Beckman Coulter J2, J6, and Avanti J series centrifuges, offering versatile use across multiple centrifuge models.

Technical Details

  • Maximum speed: 20,000 rpm
  • Critical speed range: 600 to 800 rpm
  • Density rating at max speed: 1.2 g/mL
  • Relative Centrifugal Field at max speed:
    • At rmax (108 mm): 48,400 × g
    • At rav (70 mm): 31,400 × g
    • At rmin (32 mm): 14,300 × g
  • Number of tube cavities: 8
  • Nominal tube capacity: 50 mL
  • Rotor capacity: 400 mL
  • Material: Aluminum, anodized for corrosion resistance
  • Weight (fully loaded): 7.4 kg (16.5 lb)
  • Acceleration time (fully loaded): 1 ½ minutes
  • Deceleration time (fully loaded): 2 ½ minutes
  • K factor at maximum speed: 769
  • Nominal tube dimensions (largest tube): 29 × 104 mm
  • Maximum allowable imbalance of opposing loads: 2 grams
  • Rotor entry code for J2 and J6 series centrifuges: 20.2
  • Rotor entry code for Avanti J-E centrifuges: 20
  • Rotor material: Aluminum (anodized for corrosion resistance)
  • O-ring material: Buna N rubber for maintaining atmospheric pressure during centrifugation

Compatible Accessories

  • O-rings (Buna N rubber): Spare O-rings ensure proper atmospheric sealing during centrifugation.
  • Adapters for Various Tube Sizes: Different tube adapters are available to accommodate smaller tubes or non-standard sizes.
  • Lubricant for O-rings: Ensures proper sealing and longevity of the Buna N O-rings, crucial for maintaining atmospheric pressure during runs.

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