Sequencing-Based Detection System GeneAmp 5700

GeneAmp® 5700: A Complete System for Real-Time PCR Detection

The GeneAmp® 5700 from Applied Biosystems is a fully integrated instrument designed to meet the demands of real-time quantitative PCR in molecular biology research. This all-in-one system combines several built-in components: an optical detection unit, a GeneAmp® 9600 thermal cycler, a dedicated electronics module, and a Windows NT-compatible computer, preconfigured for data processing.

The GeneAmp® 5700 is intended for use in laboratory environments that meet specific conditions for temperature (15–30 °C), humidity (≤ 80% RH), ventilation, and chemical safety. Designed to fit on compact benchtop spaces, it ensures reliable performance with a vertical configuration of its main modules, optimizing spatial efficiency.

The device requires a dedicated 2.0 kVA power supply with proper grounding and must be connected to four nearby power outlets. It is compatible with electrical standards across multiple regions (Europe, North America, Japan, Australia, etc.), with a voltage tolerance of ±10%.

The GeneAmp® 5700 uses specific PCR reagents provided in the installation kit, including SYBR Green and TaqMan DNA Template reagents, which must be stored at –20 °C. Due to the use of potentially hazardous chemicals, strict handling procedures must be followed according to Material Safety Data Sheets (MSDS), and the system should be operated in well-ventilated areas, such as under a fume hood.

Additional laboratory equipment not included with the system is required, such as PCR-compatible pipettes, 96-well plate consumables, and a data backup device.

Designed exclusively for research use only, the GeneAmp® 5700 is not intended for clinical diagnostic purposes. It offers a robust solution for quantitative PCR with fluorescence-based detection, delivering the precision and reproducibility expected in advanced scientific workflows.

- System Components:

• GeneAmp® 5700 Detector (optical unit)
• GeneAmp® 9600 Thermal Cycler
• Electronics Unit
• Computer with monitor (Windows NT compatible)

- Dimensions & Weight:

• GeneAmp® 5700 Detector: 17 cm (W) × 52 cm (D) × 30.5 cm (H) – 9.2 kg
• GeneAmp® 9600 Thermal Cycler: 52 cm (W) × 61 cm (D) × 30.5 cm (H) – 40.5 kg
• Electronics Unit: 11.5 cm (W) × 33 cm (D) × 15 cm (H) – 3.2 kg
• Computer with monitor: 41 cm (W) × 41 cm (D) × 52 cm (H) – 20 kg
• Total system weight: approx. 73 kg

- Operating Environment:

• Temperature range: 15 °C to 30 °C
• Maximum relative humidity: 80%
• Maximum altitude: 2000 meters

- Power Requirements:

• Dedicated 2.0 kVA power line with proper grounding
• 4 standard power outlets within 1.8 m of the system
• Voltage: 100 V to 240 V ±10% (region-dependent)
• Frequency: 50/60 Hz ±1%

- Thermal Output:

• Approx. 5000 BTU/h (~1500 W)
• Rear ventilation required (minimum 15 cm clearance)

- Connectivity:

• Ports: IEEE1284, RS-232, CCD, keyboard, mouse, monitor
• Interconnecting cables for detector, electronics, thermal cycler, and computer

- Safety & Compliance:

• Proper grounding is essential
• Hazard indicators: electrical, laser exposure, hot surfaces
• For laboratory use only – not intended for clinical diagnostics