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SIL-10-AF Sample Changer

Shimadzu's SIL-10-AF sample changer is designed to deliver exceptional speed and accuracy in HPLC systems. Capable of injecting volumes up to 5 mL in a standard configuration, it is ideal for preparative and analytical applications requiring high throughput and reliability. With an injection time of only 40 seconds for 10 µL, it enables rapid repeat analyses with efficient rinsing between injections. The SIL-10-AF ensures excellent repeatability, flexibility in sample preparation, and supports various vial and microplate formats to maximise laboratory productivity.



Description

Shimadzu's SIL-10-AF: The high-performance sample changer for your HPLC systems

The Shimadzu SIL-10-AF sample changer is an essential solution for laboratories looking for a device that combines speed, accuracy and flexibility. Designed to meet the most stringent requirements of high-performance liquid chromatography (HPLC) analysis, the SIL-10-AF optimises every step of sample preparation and injection. Its ingenious design significantly reduces the time between analyses while ensuring excellent result reliability, which is essential for laboratories focused on productivity and data accuracy.

One of the major advantages of the SIL-10-AF sample changer is its speed. The injection time is only 40 seconds for a volume of 10 µL, and with the flow line rinsing included, the device is ready for the next injection in approximately two minutes. This speed ensures a high analysis throughput, which is essential for environments requiring numerous daily tests. Combined with a processing capacity of 192 samples with two 96-well microtitre plates, it is equally suited to small analytical volumes and semi-preparative needs.

Designed for versatility, the SIL-10-AF offers a wide range of injection volumes, from 1 µL to 5,000 µL with additional options. This flexibility makes it suitable for both conventional analyses and applications requiring sample enrichment or larger volumes for LC preparations. Its ability to handle different vial formats, from microtitre plates to 13 mL vials, offers valuable adaptability depending on experimental protocols and volume requirements.

Accuracy and repeatability are essential criteria for any quantitative analysis, and the SIL-10-AF excels in these areas. Its accuracy for an injection volume of 10 µL is remarkable, with a maximum relative standard deviation (RSD) of 0.5% in standard mode. In addition, the system effectively limits cross-contamination through the use of an optional fixed loop injection, ensuring reliable and reproducible results even when using different sample types.

Ease of use is also at the heart of the SIL-10-AF's design. With optimal temperature control (from 4°C to 70°C thanks to an optional cooler), it ensures ideal preservation of sensitive samples. Acidity management is equally rigorous, with an operating range from pH 1 to pH 10. Finally, its compact dimensions and moderate weight allow it to be easily integrated into various laboratory environments without requiring additional space.

Features

  • Fast injection in 40 seconds for 10 µL
  • Sample preparation with dilution and reagent addition
  • Excellent linearity and repeatability for quantitative analysis
  • Compatible with different vial and plate formats
  • Configurable needle rinsing before and after injection
  • Controlled sample storage temperature
  • Suitable for analytical and semi-preparative applications
  • Large volume injection possible up to 5 mL
  • Effective reduction of carryover between samples


Technical Details

  • Injection method: Fixed loop injection, variable injection volume
  • Injection volume range: 1 µL to 50 µL (standard), up to 5,000 µL (with options)
  • Sample capacity:
    • 100 x 1.5 mL vials (60 with cooler)
    • 80 x 4 mL vials (50 with cooler)
    • 25 x 13 mL vials
    • 192 samples on two 96-well plates
  • Injection volume accuracy: RSD ≤ 0.5% (10 µL, standard mode)
  • Cross-contamination: Minimised with fixed loop injection
  • Maximum number of repeat injections: 30 per sample
  • Operating pH range: pH 1 to pH 10
  • Operating temperature range: 4 °C to 35 °C
  • Main dimensions:
    • Main unit: 260 mm (W) x 280 mm (H) x 420 mm (D), 19 kg
    • Syringe unit: 100 mm (W) x 280 mm (H) x 150 mm (D), 4 kg
  • Power supply: AC 110V or 230V, 100VA, 50/60Hz

Compatible Accessories

  • Sample cooler type S (228-45063-xx) or L (228-45064-xx)
  • Racks for 1.5 mL, 4 mL and 13 mL vials
  • Racks for 96-well microtiter plates
  • Extension kits for injection volumes up to 400 µL, 2,000 µL or 5,000 µL
  • Syringe module for variable volume injection


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