Simoa HD-1: a precision tool for detecting very low concentrations of biomarkers
The Simoa HD-1 Analyzer from Quanterix is a digital immunoassay analyser that pushes the limits of biomarker detection by achieving femtomolar concentrations. It is based on Simoa (Single Molecule Array) technology, a digitalised version ofthe classicELISA. This method involves trapping and encapsulating individual immunocomplexes on paramagnetic beads, which are inserted into wells measuring just a few femtolitres, organised on Simoa discs. Each well is then analysed to determine whether or not it contains a target molecule, producing a binary "on/off" reading that is then translated into a precise concentration.
With an average sensitivity 1,000 times higher than conventional methods, the Simoa HD-1 enables the detection of previously inaccessible markers. It is particularly useful for research where sample volumes are limited, as it remains effective even after dilution. The analyser incorporates a comprehensive automation system from sample loading to result reading, significantly reducing human error and handling time.
The touchscreen interface allows intuitive operation in five simple steps: select the assay, load the reagents, samples and consumables, and start the analysis. The first reading is available in just 30 minutes, with a reading rate of 45 seconds per additional result. Each Simoa disc contains 24 sets of 216,000 micro-wells, ensuring reproducible performance for each sample.
The Simoa HD-1 is also compatible with Quanterix ready-to-use kits and custom protocols (homebrew), offering great flexibility in assay design. It can process up to four analytes simultaneously (4-plex), while maintaining accuracy with coefficients of variation below 10%. With a dynamic range of over 4 logs, it combines digital measurements for low concentrations and analogue measurements for higher concentrations.
Used in key areas such as neurology, oncology, infectious diseases and inflammatory diseases, the Simoa HD-1 allows researchers to explore the frontiers of precision medicine with consistent reliability. Its compact size, accuracy and low operating cost make it the tool of choice for academic and industrial laboratories seeking to maximise the value of their biomolecular research.
