Spark 10M Microplate Reader - Perfect Condition

Spark 10M: Multimode reader designed for cellular applications

The Spark 10M is specifically designed to meet the demands of modern cell applications. With its integrated gas control module (GCM™), it enables automated CO₂ and O₂ regulation, ensuring stable culture conditions and improved cell viability. This feature extends experiment times without compromising results. In addition, the patented humidity cassette reduces evaporation in standard microplates, allowing for extended kinetic studies without the need for expensive plates.

The Spark 10M also features an automated lid lifting system (Lid Lifter™), facilitating reagent additions during experiments and optimising gas exchange. These combined features ensure complete and reliable automation for long-term assays.

Versatile, high-precision detection for all applications

This reader offers a comprehensive range of detection modes: absorbance, fluorescence (intensity, TRF, FRET, TR-FRET, polarisation), luminescence (flash, glow, multi-colour), and Alpha Technology (AlphaScreen®, AlphaLISA®, AlphaPlex™). Its high-speed monochromator (HSM) enables ultra-fast absorbance measurements across the entire spectrum (200–1000 nm) in less than five seconds, with exceptional accuracy, particularly in the deep UV for DNA and protein quantification.

The Spark 10M's Fusion Optics technology allows independent selection of filters and monochromators for excitation and emission, offering maximum flexibility and sensitivity for each well and each assay. Multi-colour luminescence measurements are optimised with 40 selectable filters and an extended dynamic range.

SparkControl™ software and intelligent automation for increased productivity

SparkControl™ software features an intuitive, touch-optimised interface that simplifies everyday lab tasks. It enables remote control of the instrument, real-time monitoring of measurements, and adjustment of environmental parameters during assays. One-click applications speed up workflows, from cell counting to nucleic acid quantification.

SparkControl Magellan™ completes the experience with powerful data analysis tools, including curve fitting, kinetic calculations, and compliance with FDA 21 CFR part 11 standards. Qualitative and quantitative data analysis is facilitated by features such as 3D scanning, background correction, and peak identification.

• Absorbance: 200–1000 nm, xenon flash lamp, OD 0–4, accuracy ≤0.5%, speed ≤5 sec
• Fluorescence: 230–900 nm (excitation), 280–900 nm (emission), detection ≤0.25 pM
• Fluorescence polarisation: 300–850 nm, detection ≤1.5 mP
• TRF: detection ≤40 fM
• Luminescence: dynamic range >9 orders of magnitude, detection ≤218 fM
• Alpha Technology: detection ≤100 amol/well
• Cell counting: size 4–90 µm, accuracy ±10%, speed <30 sec
• Gas control: CO₂ 0.04–10%, O₂ 0.1–21%, accuracy <1%
• Injectors: volumes 5–1000 µl, accuracy ≤0.5%
• Temperature control: up to 42 °C, uniformity <0.5 °C
• Agitation: linear, orbital, dual-orbital
• Plate formats: 1–384 wells, NanoQuant Plate™, Cell Chips™, cuvettes