Spectral confocal microscope TCS SP5

Leica TCS SP5: The Benchmark in Confocal Microscopy

The Leica TCS SP5 is a high-end spectral confocal microscope designed to address the growing needs of researchers in fluorescence microscopy and live sample analysis. It stands out with its prism spectrometer, which enables maximum light transmission while providing unmatched flexibility in spectral band selection. Its tandem scanning system integrates both a conventional and a resonant scanner, making it ideal for both high-resolution static image acquisition and fast imaging of dynamic processes.

The TCS SP5 supports up to five simultaneous confocal channels, enabling multi-fluorescence imaging with unparalleled precision. Its AOBS® module replaces traditional dichroic mirrors, offering maximum light transmission and immediate adaptability to new laser wavelengths or fluorochromes. This makes it an ideal solution for laboratories aiming to stay at the forefront of advances in confocal microscopy.

One of the major strengths of the Leica TCS SP5 is its ability to perform rapid acquisitions thanks to its resonant scanner, which can capture up to 200 images per second. It is also equipped with non-descanned detectors (NDD) optimized for multiphoton microscopy, ensuring efficient detection of weak signals and finer analysis of biological phenomena in-depth.

With an intuitive user interface and software assistants for techniques such as FRAP (Fluorescence Recovery After Photobleaching), FRET (Fluorescence Resonance Energy Transfer), and FLIM (Fluorescence Lifetime Imaging Microscopy), the Leica TCS SP5 is both powerful and user-friendly. Its modular system allows adaptation to various applications, from cell biology to neurobiology and pharmacology.

The Leica TCS SP5 is the ideal solution for researchers seeking fast, flexible, and precise confocal imaging. Its modular architecture allows it to be adapted to all needs in advanced microscopy.

• Compatible Microscopes: Leica DM5000 CS, DM6000 CS (upright), and DMI 6000 CS (inverted)
• Z-drive: SuperZ galvanometer stage, 1500 µm travel range with 3 nm precision
• Lasers:
  • VIS diode (18 mW, 442 nm)
  • Argon (100 mW, 458, 476, 488, 496, 514 nm)
  • HeNe (1 mW 543 nm, 2 mW 594 nm, 10 mW 633 nm)
  • DPSS (10 mW, 561 nm)
  • UV diode (50 mW, 405 nm)
  • Ti:Sapphire pulsed (1.2 ps, 1 W, 720-1000 nm)
• Detection:
  • Detection range: 400-800 nm
  • Number of spectral channels: 5
  • Emission band tunability: 1 nm steps
  • Detectors: High-sensitivity, low-noise PMT
• Scanner:
  • Conventional mode: 8192 x 8192 pixels, up to 5 Hz (512 x 512)
  • Resonant mode: up to 250 Hz (512 x 16)
  • Optical field rotation: 200°