5825747003: 12 × 75 mm Adapter (5 mL FACS) for Standardised Clinical Flow
The Eppendorf 5825747003 is the dedicated adapter for 12 × 75 mm, 5 mL FACS tubes, a reference format in cytometry and clinical analysis. Its round bottom geometry holds the tube securely in place and minimises local stress during acceleration and braking, while supporting routine procedures in hospital and private laboratories. Each adapter holds 27 cuvettes, allowing up to 108 positions per rotor when the configuration allows.
Proven Compatibility with Eppendorf Rotors
The 5825747003 fits into the S-4-104 (for 5810/5810 R centrifuges ), S-4x750 (for 5910 Ri/5920 R centrifuges) and S-4x1000 (for 5920 R centrifuges) rotors. This compatibility ensures continuity of methods between platforms, which is useful for multi-site laboratories or technical platforms that operate several centrifuge models. The tube side dimensions are strictly defined: max. diameter 12 mm, and max. bowl heights vary depending on the rotor (e.g. up to 121 mm on S-4x750/S-4x1000, with 113 mm when working with the lid in place). These limits facilitate consumable selection and compliance with SOPs.
Performance and Parameters per Rotor
As with any adapter, performance depends on the host rotor. On the S-4-104 (5810/5810 R), we typically work with a radius of ≈ 183 mm and a max RCF of ≈ 3,112 × g for this adapter position. On S-4x750 (5910 Ri/5920 R), ≈ 4,594 × g is achieved with a radius of ≈ 186 mm. On S-4x1000 (5920 R), the reference values are radius 211 mm and max RCF 3,229 × g for the 12 × 75 mm column. Observing the usable heights and performing a tilt test if the length of the tubes approaches the limit ensures that there is no contact with the rotor head.
